Anti-FOXO1A antibody (ab39670)
Key features and details
- Rabbit polyclonal to FOXO1A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A -
Host species
Rabbit -
Specificity
ab39670 detects endogenous levels of total FOXO1A. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human FOXO1A. Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.
Database link: Q12778
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Corresponding phospho antibody
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab39670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
- SwissProt: G3V7R4 Rat
- Unigene: 370666 Human
see all -
Alternative names
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
see all
Images
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Western blot - Anti-FOXO1A antibody (ab39670)Lanes 1-3 : Anti-FOXO1A antibody (ab39670) at 1/500 dilution
Lanes 4-5 : Anti-FOXO1A (phospho S319) antibody (ab38516) at 1/500 dilution
Lane 1 : 293 Cell extract
Lanes 2-5 : HeLa Cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 70 kDa
Observed band size: 70 kDa10% gel.
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight.
Washing condition: 5 ml TBST, 4 x 5min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Chen, C. et al PLoS One. 2013 Jul 9;8(7):e69260. doi: 10.1371/journal.pone.0069260. Print 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Tissue microarray analysis of FOXO1 expression
Representative images of FOXO1 expression in normal brain tissue (C, ×200); grade II diffuse astrocytoma (D, ×200); grade III anaplastic astrocytoma (E, ×200) and grade IV glioblastoma (F, ×200).
FOXO1 was detected using ab39670 at 1/200 dilution. The tissue microarray was contructed from paraffin-embedded human brain tissue samples.
(After Figure 1 of Chen et al).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Paraffin-embedded human brain tissue stained for FOXO1 with ab39670 at 1/50 dilution in immunohistochemical analysis. The right hand image was obtained following blocking with the immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39670. (Left image: without peptide; Right image: with peptide 1µg/ml). -
Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody (ab39670)ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-FOXO1A antibody (ab39670)This image is courtesy of an anonymous abreview.Anti-FOXO1A antibody (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
Exposure time: 30 secondsPrimary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.
Protocols
References (53)
ab39670 has been referenced in 53 publications.
- Ding HR et al. Protective Properties of FOXO1 Inhibition in a Murine Model of Non-alcoholic Fatty Liver Disease Are Associated With Attenuation of ER Stress and Necroptosis. Front Physiol 11:177 (2020). PubMed: 32218743
- Zhang F et al. Obesity-induced overexpression of miR-802 impairs insulin transcription and secretion. Nat Commun 11:1822 (2020). PubMed: 32286278
- Wu YJ et al. Danzhi Jiangtang Capsule Mediates NIT-1 Insulinoma Cell Proliferation and Apoptosis by GLP-1/Akt Signaling Pathway. Evid Based Complement Alternat Med 2019:5356825 (2019). PubMed: 31467576
- Huang F et al. FoxO1-mediated inhibition of STAT1 alleviates tubulointerstitial fibrosis and tubule apoptosis in diabetic kidney disease. EBioMedicine 48:491-504 (2019). PubMed: 31629675
- Li X et al. Hsa_circ_0002483 inhibited the progression and enhanced the Taxol sensitivity of non-small cell lung cancer by targeting miR-182-5p. Cell Death Dis 10:953 (2019). PubMed: 31844042
