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    foxo1a-antibody-ab39670.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors
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Anti-FOXO1A antibody (ab39670)

  • Datasheet
  • SDS
Reviews (12)Q&A (3)References (53)

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Western blot - Anti-FOXO1A antibody (ab39670)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody (ab39670)
  • Western blot - Anti-FOXO1A antibody (ab39670)

Key features and details

  • Rabbit polyclonal to FOXO1A
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FOXO1A antibody
    See all FOXO1A primary antibodies
  • Description

    Rabbit polyclonal to FOXO1A
  • Host species

    Rabbit
  • Specificity

    ab39670 detects endogenous levels of total FOXO1A.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human FOXO1A. Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.
    Database link: Q12778

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Assay kits

    • FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Corresponding phospho antibody

    • Anti-FOXO1A (phospho S319) antibody (ab47326)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa membrane extract lysate (ab29547)

Applications

Our Abpromise guarantee covers the use of ab39670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Target information above from: UniProt accession Q12778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2308 Human
    • Entrez Gene: 56458 Mouse
    • Entrez Gene: 84482 Rat
    • Omim: 136533 Human
    • SwissProt: Q12778 Human
    • SwissProt: Q9R1E0 Mouse
    • SwissProt: G3V7R4 Rat
    • Unigene: 370666 Human
    • Unigene: 29891 Mouse
    • Unigene: 116108 Rat
    see all
  • Alternative names

    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Images

  • Western blot - Anti-FOXO1A antibody (ab39670)
    Western blot - Anti-FOXO1A antibody (ab39670)
    Lanes 1-3 : Anti-FOXO1A antibody (ab39670) at 1/500 dilution
    Lanes 4-5 : Anti-FOXO1A (phospho S319) antibody (ab38516) at 1/500 dilution

    Lane 1 : 293 Cell extract
    Lanes 2-5 : HeLa Cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    10% gel.

    Running conditions: 60v, 30min; 120v 60min

    Transfer conditions: 150mA 120min Nitrocellulose membrane.

    Blocking conditions: 5% non-fat milk in TBST, RT, 90min.

    Primary antibody incubation: 4?, overnight.

    Washing condition: 5 ml TBST, 4 x 5min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Chen, C. et al PLoS One. 2013 Jul 9;8(7):e69260. doi: 10.1371/journal.pone.0069260. Print 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Tissue microarray analysis of FOXO1 expression

    Representative images of FOXO1 expression in normal brain tissue (C, ×200); grade II diffuse astrocytoma (D, ×200); grade III anaplastic astrocytoma (E, ×200) and grade IV glioblastoma (F, ×200).

    FOXO1 was detected using ab39670 at 1/200 dilution. The tissue microarray was contructed from paraffin-embedded human brain tissue samples.

    (After Figure 1 of Chen et al).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)

    Paraffin-embedded human brain tissue stained for FOXO1 with ab39670 at 1/50 dilution in immunohistochemical analysis. The right hand image was obtained following blocking with the immunizing peptide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39670. (Left image: without peptide; Right image: with peptide 1µg/ml).
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody (ab39670)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody (ab39670)

    ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-FOXO1A antibody (ab39670)
    Western blot - Anti-FOXO1A antibody (ab39670)This image is courtesy of an anonymous abreview.
    Anti-FOXO1A antibody (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa
    Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)


    Exposure time: 30 seconds


    Primary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (53)

    Publishing research using ab39670? Please let us know so that we can cite the reference in this datasheet.

    ab39670 has been referenced in 53 publications.

    • Ding HR  et al. Protective Properties of FOXO1 Inhibition in a Murine Model of Non-alcoholic Fatty Liver Disease Are Associated With Attenuation of ER Stress and Necroptosis. Front Physiol 11:177 (2020). PubMed: 32218743
    • Zhang F  et al. Obesity-induced overexpression of miR-802 impairs insulin transcription and secretion. Nat Commun 11:1822 (2020). PubMed: 32286278
    • Wu YJ  et al. Danzhi Jiangtang Capsule Mediates NIT-1 Insulinoma Cell Proliferation and Apoptosis by GLP-1/Akt Signaling Pathway. Evid Based Complement Alternat Med 2019:5356825 (2019). PubMed: 31467576
    • Huang F  et al. FoxO1-mediated inhibition of STAT1 alleviates tubulointerstitial fibrosis and tubule apoptosis in diabetic kidney disease. EBioMedicine 48:491-504 (2019). PubMed: 31629675
    • Li X  et al. Hsa_circ_0002483 inhibited the progression and enhanced the Taxol sensitivity of non-small cell lung cancer by targeting miR-182-5p. Cell Death Dis 10:953 (2019). PubMed: 31844042
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 15 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-FOXO1A antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain, Hippocampus)
    Specification
    Brain, Hippocampus
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 04 2020

    Western blot abreview for Anti-FOXO1A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole brain tissue lysate)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
    Loading amount
    20 µg
    Specification
    Whole brain tissue lysate
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 31 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (vessel)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    No
    Specification
    vessel
    Blocking step
    h2o2, then serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FOXO1A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (primary lymphoma cells)
    Permeabilization
    Yes - 0.2 % TritonX in PBS, 5 min
    Specification
    primary lymphoma cells
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 09 2017

    Western blot abreview for Anti-FOXO1A antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (primary lymphoma)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    primary lymphoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 09 2017

    Western blot abreview for Anti-FOXO1A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (chondrocytes)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    chondrocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Fixative
    zinc buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 23 2015

    Western blot abreview for Anti-FOXO1A antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Caenorhabditis elegans Cell lysate - whole cell (whole worm lysate)
    Loading amount
    80 µg
    Specification
    whole worm lysate
    Gel Running Conditions
    Reduced Denaturing (SDS-Page 10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Lorenzo Ramos-Mucci

    Verified customer

    Submitted Dec 12 2012

    Question

    Hello,
    I am calling in regards to an order for 'Anti-FOXO1A antibody - ChIP Grade' I placed. Although the catalog number is correct (ab39670), the product description reads "Rb pAb to Fox01A." Could you please explain this discrepancy to me?
    Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 03 2012

    Answer

    Thank you for contacting Abcam.

    I confirmed that the product sent to you was ab39670, "Anti-FOXO1A antibody - ChIP grade." This antibody is a rabbit (Rb) polyclonal (pAb) to FOXO1A, hence the name "Rb pAb to Fox01A."

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 03 2012

    Western blot abreview for Anti-FOXO1A antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LNCaP)
    Loading amount
    40 µg
    Specification
    LNCaP
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 30 2012

    1-10 of 15 Abreviews or Q&A

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