Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP)
Loading amount
40 µg
Specification
LNCaP
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 25, 90 kDa
Additional data
Additional Notes
Few additinal non-specific bands
Abcam user community
Verified customer
Submitted Mar 30 2012