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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors
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RecombinantRabMAb

Recombinant Anti-FOXO1A antibody [EP927Y] (ab52857)

  • Datasheet
Reviews (6) Submit a question References (49)

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Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Flow Cytometry - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Anti-FOXO1A antibody [EP927Y] (ab52857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP927Y] to FOXO1A
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Human

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Primary
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Assay
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FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)

View more associated products

Overview

  • Product name

    Anti-FOXO1A antibody [EP927Y]
    See all FOXO1A primary antibodies
  • Description

    Rabbit monoclonal [EP927Y] to FOXO1A
  • Host species

    Rabbit
  • Specificity

    ab52857 recognizes both phosphorylated and unphosphorylated FOXO1A. The immunogen used for this product shares 85% homology with FOXO3. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human FOXO1A (phospho S329). The exact sequence is proprietary.

  • Positive control

    • WB: NIH/3T3 cell lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP927Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-FOXO1A antibody [EP927Y] (ab200694)
    • Alexa Fluor® 647 Anti-FOXO1A antibody [EP927Y] (ab200695)
    • Alexa Fluor® 594 Anti-FOXO1A antibody [EP927Y] (ab207242)
    • Alexa Fluor® 555 Anti-FOXO1A antibody [EP927Y] (ab207244)
    • Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
  • Assay kits

    • FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 cytoplasmic extract lysate (ab14873)
    • NIH/3T3 nuclear extract lysate (ab14874)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Mouse
All applications
Rat
Application Abreviews Notes
IHC-P (1)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/90.
WB (5)
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
Notes
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/90.
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
    • Target information above from: UniProt accession Q12778 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2308 Human
      • Entrez Gene: 56458 Mouse
      • Entrez Gene: 84482 Rat
      • Omim: 136533 Human
      • SwissProt: Q12778 Human
      • SwissProt: Q9R1E0 Mouse
      • SwissProt: G3V7R4 Rat
      • Unigene: 370666 Human
      • Unigene: 29891 Mouse
      • Unigene: 116108 Rat
      see all
    • Alternative names

      • FKH 1 antibody
      • FKH1 antibody
      • FKHR antibody
      • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
      • Forkhead box O1 antibody
      • Forkhead box protein O1 antibody
      • Forkhead box protein O1A antibody
      • Forkhead in rhabdomyosarcoma antibody
      • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
      • FoxO transcription factor antibody
      • foxo1 antibody
      • FOXO1_HUMAN antibody
      • FOXO1A antibody
      • OTTHUMP00000018301 antibody
      see all

    Images

    • Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
      Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
      Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 70 kDa
      Observed band size: 70 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)

      ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
      Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
      ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.
    • Flow Cytometry - Anti-FOXO1A antibody [EP927Y] (ab52857)
      Flow Cytometry - Anti-FOXO1A antibody [EP927Y] (ab52857)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Anti-FOXO1A antibody [EP927Y] (ab52857)
      Anti-FOXO1A antibody [EP927Y] (ab52857)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (49)

    Publishing research using ab52857? Please let us know so that we can cite the reference in this datasheet.

    ab52857 has been referenced in 49 publications.

    • Zhang J  et al. Down-regulation of LINC00472 promotes osteosarcoma tumorigenesis by reducing FOXO1 expressions via miR-300. Cancer Cell Int 20:100 (2020). PubMed: 32256209
    • Zhang J  et al. N1-Methylnicotinamide Improves Hepatic Insulin Sensitivity via Activation of SIRT1 and Inhibition of FOXO1 Acetylation. J Diabetes Res 2020:1080152 (2020). PubMed: 32280711
    • El-Merahbi R  et al. The adrenergic-induced ERK3 pathway drives lipolysis and suppresses energy dissipation. Genes Dev 34:495-510 (2020). PubMed: 32139423
    • Tian F  et al. MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver. Yonsei Med J 61:780-788 (2020). PubMed: 32882762
    • Shu Q  et al. Acupuncture Targeting SIRT1 in the Hypothalamic Arcuate Nucleus Can Improve Obesity in High-Fat-Diet-Induced Rats with Insulin Resistance via an Anorectic Effect. Obes Facts 13:40-57 (2020). PubMed: 31935731
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-FOXO1A antibody [EP927Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (SW480 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20 Tris Glycin Gel)
    Loading amount
    50 µg
    Specification
    SW480 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 17 2018

    Western blot abreview for Anti-FOXO1A antibody [EP927Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver Extract)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycin)
    Loading amount
    20 µg
    Specification
    Liver Extract
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2017

    Western blot abreview for Anti-FOXO1A antibody [EP927Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Specification
    human cell
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody [EP927Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
    Permeabilization
    Yes - 0.025% Triton x-100
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Western blot abreview for Anti-FOXO1A antibody [EP927Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    36 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Rat Tissue lysate - whole (Bone - Tibia)
    Specification
    Bone - Tibia
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Mr. Helder Fonseca

    Verified customer

    Submitted Sep 25 2014

    Western blot abreview for Anti-FOXO1A [EP927Y] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth musle cells)
    Specification
    rat vascular smooth musle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

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