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    foxo1a-antibody-ep927y-ab52857.pdf

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RecombinantRabMAb

Recombinant Anti-FOXO1A antibody [EP927Y] (ab52857)

  • Datasheet
Reviews (8) Submit a question References (63)

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Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Flow Cytometry (Intracellular) - Anti-FOXO1A antibody [EP927Y] (ab52857)
  • Anti-FOXO1A antibody [EP927Y] (ab52857)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP927Y] to FOXO1A
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

You may also be interested in

Primary
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Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] (ab154786)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Kit
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FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)

View more associated products

Overview

  • Product name

    Anti-FOXO1A antibody [EP927Y]
    See all FOXO1A primary antibodies
  • Description

    Rabbit monoclonal [EP927Y] to FOXO1A
  • Host species

    Rabbit
  • Specificity

    ab52857 recognizes both phosphorylated and unphosphorylated FOXO1A. The immunogen used for this product shares 85% homology with FOXO3. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 cell lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP927Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXO
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-FOXO1A antibody [EP927Y] (ab200694)
    • Alexa Fluor® 647 Anti-FOXO1A antibody [EP927Y] (ab200695)
    • Alexa Fluor® 594 Anti-FOXO1A antibody [EP927Y] (ab207242)
    • Alexa Fluor® 555 Anti-FOXO1A antibody [EP927Y] (ab207244)
    • Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
  • Assay kits

    • FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 nuclear extract lysate (ab14874)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/90.
IHC-P (3)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
WB (5)
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
Notes
Flow Cyt (Intra)
1/90.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
Application notes
Is unsuitable for IP.

Target

  • Function

    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications

    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Target information above from: UniProt accession Q12778 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2308 Human
    • Entrez Gene: 56458 Mouse
    • Entrez Gene: 84482 Rat
    • Omim: 136533 Human
    • SwissProt: Q12778 Human
    • SwissProt: Q9R1E0 Mouse
    • SwissProt: G3V7R4 Rat
    • Unigene: 370666 Human
    • Unigene: 29891 Mouse
    see all
  • Alternative names

    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Images

  • Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
    Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)
    Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)

    ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)
    ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.
  • Flow Cytometry (Intracellular) - Anti-FOXO1A antibody [EP927Y] (ab52857)
    Flow Cytometry (Intracellular) - Anti-FOXO1A antibody [EP927Y] (ab52857)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

     

     

  • Anti-FOXO1A antibody [EP927Y] (ab52857)
    Anti-FOXO1A antibody [EP927Y] (ab52857)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (63)

Publishing research using ab52857? Please let us know so that we can cite the reference in this datasheet.

ab52857 has been referenced in 63 publications.

  • Jing D  et al. FoxO1a mediated cadmium-induced annulus fibrosus cells apoptosis contributes to intervertebral disc degeneration in smoking. J Cell Physiol 236:677-687 (2021). PubMed: 32654176
  • Zhang L  et al. Role and mechanism underlying FoxO6 in skeletal muscle in vitro and in vivo. Int J Mol Med 48:N/A (2021). PubMed: 34080654
  • Liu X  et al. Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1. Cancer Cell Int 21:43 (2021). PubMed: 33430878
  • Li D  et al. miR-498 inhibits autophagy and M2-like polarization of tumor-associated macrophages in esophageal cancer via MDM2/ATF3. Epigenomics 13:1013-1030 (2021). PubMed: 34114479
  • Yang H  et al. Fluoxetine regulates glucose and lipid metabolism via the PI3K-AKT signaling pathway in diabetic rats. Mol Med Rep 22:3073-3080 (2020). PubMed: 32945450
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody [EP927Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16 Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Specification
B16 Pancreas
Blocking step
Bloxall as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 31 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody [EP927Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Panc1 Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
Permeabilization
No
Specification
Panc1 Tumor
Blocking step
Bloxall as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 30 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody [EP927Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
Yes - 0.025% Triton x-100
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 09 2015

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