Name: Anti-FOXO1A antibody [EP927Y]
Brand: RabMAb
SKU: ab52857
Rating: 5 (6 reviews)

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Primary - Rabbit Anti-FOXO1A antibody [EP927Y] (ab52857)
IHC-P, ICC/IF, Flow Cyt, WB

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

View more associated products

Product name

Anti-FOXO1A antibody [EP927Y]
See all FOXO1A primary antibodies
Description

Rabbit monoclonal [EP927Y] to FOXO1A
Host species

Rabbit
Specificity

ab52857 recognizes both phosphorylated and unphosphorylated FOXO1A. The immunogen used for this product shares 85% homology with FOXO3. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applications

Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human FOXO1A (phospho S329). The exact sequence is proprietary.
Positive control
- WB: NIH/3T3 cell lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP927Y
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab52857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.
Flow Cyt		1/90.
WB		1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).

Application notes

Is unsuitable for IP.

Function

Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Tissue specificity

Ubiquitous.
Involvement in disease

Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Sequence similarities

Contains 1 fork-head DNA-binding domain.
Post-translational
modifications

Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization

Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Target information above from: UniProt accession Q12778 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2308 Human
- Entrez Gene: 56458 Mouse
- Entrez Gene: 84482 Rat
- Omim: 136533 Human
- SwissProt: Q12778 Human
- SwissProt: Q9R1E0 Mouse
- SwissProt: G3V7R4 Rat
- Unigene: 370666 Human
- Unigene: 29891 Mouse
- Unigene: 116108 Rat
see all
Alternative names
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
- Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
- Forkhead box O1 antibody
- Forkhead box protein O1 antibody
- Forkhead box protein O1A antibody
- Forkhead in rhabdomyosarcoma antibody
- Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
- FoxO transcription factor antibody
- foxo1 antibody
- FOXO1_HUMAN antibody
- FOXO1A antibody
- OTTHUMP00000018301 antibody
see all

Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)

Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)

ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)

ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.
Flow Cytometry - Anti-FOXO1A antibody [EP927Y] (ab52857)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Datasheet

This product has been referenced in:

He F et al. Low-dose lipopolysaccharide inhibits neuronal apoptosis induced by cerebral ischemia/reperfusion injury via the PI3K/Akt/FoxO1 signaling pathway in rats. Mol Med Rep 19:1443-1452 (2019). Read more (PubMed: 30628689) »
Park MK et al. PTEN self-regulates through USP11 via the PI3K-FOXO pathway to stabilize tumor suppression. Nat Commun 10:636 (2019). Read more (PubMed: 30733438) »

See all 41 Publications for this product

Publishing research using ab52857? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Tissue lysate - whole (SW480 cells)

Gel Running Conditions

Reduced Denaturing (4-20 Tris Glycin Gel)

Loading amount

50 µg

Specification

SW480 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 17 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Liver Extract)

Gel Running Conditions

Reduced Denaturing (4-20% Tris-Glycin)

Loading amount

20 µg

Specification

Liver Extract

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 25 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (human cell)

Gel Running Conditions

Reduced Denaturing

Loading amount

100 µg

Specification

human cell

Blocking step

BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Lung)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0

Permeabilization

Yes - 0.025% Triton x-100

Specification

Lung

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 09 2015

Application

Western blot

Loading amount

36 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Rat Tissue lysate - whole (Bone - Tibia)

Specification

Bone - Tibia

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Helder Fonseca

Verified customer

Submitted Sep 25 2014

Application

Western blot

Loading amount

100000 cells

Gel Running Conditions

Reduced Denaturing

Sample

Rat Cell lysate - whole cell (rat vascular smooth musle cells)

Specification

rat vascular smooth musle cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 07 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Positive Controls

Applications

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-FOXO1A antibody [EP927Y]

Western blot abreview for Anti-FOXO1A antibody [EP927Y]

Western blot abreview for Anti-FOXO1A antibody [EP927Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXO1A antibody [EP927Y]

Western blot abreview for Anti-FOXO1A antibody [EP927Y]

Western blot abreview for Anti-FOXO1A [EP927Y] antibody

Post-translational
modifications