Product nameAnti-FOXO1A antibody [EP927Y] - BSA and Azide free
See all FOXO1A primary antibodies
DescriptionRabbit monoclonal [EP927Y] to FOXO1A - BSA and Azide free
Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to residues surrounding Ser329 of human FOXO1A.
- NIH/3T3 cell lysate, human bladder carcinoma tissue and HeLa cells.
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 488) (ab200694)
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 647) (ab200695)
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 594) (ab207242)
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 405) (ab207243)
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 555) (ab207244)
- Anti-FOXO1A antibody [EP927Y] (Alexa Fluor® 568) (ab207245)
- Anti-FOXO1A antibody [EP927Y] (ab52857)
Our Abpromise guarantee covers the use of ab219734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).|
FunctionTranscription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
Involvement in diseaseDefects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsPhosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
- Information by UniProt
- FKH 1 antibody
- FKH1 antibody
- FKHR antibody
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52857).
This product has been referenced in:
- Pomiès P et al. Involvement of the FoxO1/MuRF1/Atrogin-1 Signaling Pathway in the Oxidative Stress-Induced Atrophy of Cultured Chronic Obstructive Pulmonary Disease Myotubes. PLoS One 11:e0160092 (2016). Read more (PubMed: 27526027) »
- Shao D et al. A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response. Nat Commun 5:3315 (2014). Read more (PubMed: 24525530) »