• Product name
    Anti-FOXO1A (phospho S256) antibody
    See all FOXO1A primary antibodies
  • Description
    Rabbit polyclonal to FOXO1A (phospho S256)
  • Host species
  • Specificity
    ab131339 detects FOXO1A only when phosphorylated at serine 256
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the sequence around phosphorylation site of serine 256 (


    ) of Human FOXO1A (NP_002006.2).

  • Positive control
    • MCF7 cells, 293 cell extracts, Human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab131339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.


  • Function
    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificity
  • Involvement in disease
    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all


  •  Immunofluorescent analysis of formalin-fixed, paraffin-embedded human breast tissue with Anti-FOXO1A at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

  • All lanes : Anti-FOXO1A (phospho S256) antibody (ab131339) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts treated with serum

    Predicted band size: 69 kDa

  • Immunofluorescence analysis of methanol-fixed MCF7 cells using ab131339 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131339 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.


This product has been referenced in:
  • Feng YL  et al. Resveratrol prevents osteoporosis by upregulating FoxO1 transcriptional activity. Int J Mol Med 41:202-212 (2018). Read more (PubMed: 29115382) »
  • Du YN  et al. SGK1-FoxO1 Signaling Pathway Mediates Th17/Treg Imbalance and Target Organ Inflammation in Angiotensin II-Induced Hypertension. Front Physiol 9:1581 (2018). Read more (PubMed: 30524295) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (Human fibroblast and stem cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Human fibroblast and stem cell
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 06 2017

Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer
Yes - 0.2% Triton X-100
Brain Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 26 2016

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain)
Yes - 0.2% Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Muscle)
Gel Running Conditions
Reduced Denaturing (3-8%)
Loading amount
30 µg
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 04 2016

Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Human Cell (muscle)
Yes - Triton

Abcam user community

Verified customer

Submitted Dec 03 2014


Thank you for contacting Abcam regarding the FOXO1A antibodies ab39656 and ab131339.

I have examined the homology between the immunogen sequences and the bovine sequence, and they are actually identical. In theory then, these antibodies should be able to recognize the bovine protein. Since we have not tested this the antibodies are not guaranteed to work with this species. Further optimization may be required.

I have check some of our other antibodies to this target and would like to propose the following alternative:

ab38501 - Anti-FOXO1A (phospho S256) antibody. The immunogen is a bit longer and may confer greater specificity, resulting in less background.

Since ab38501 has not been tested in bovine in WB, I can offer a discount off a future purchase if you buy ab38501 now, test it inbovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab38501 in bovine in WB. I will then send a discount code. This code must be issued before purchasing ab38501 so please wait for my reply before ordering.

2. Purchase ab38501 either by phone, fax, or online (www.abcam.com).

3. Test it in bovine in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab38501 turns out to be unsuitable for bovine in WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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