Overview

  • Product name
    Anti-FOXO1A (phospho S256) antibody
    See all FOXO1A primary antibodies
  • Description
    Rabbit polyclonal to FOXO1A (phospho S256)
  • Host species
    Rabbit
  • Specificity
    ab131339 detects FOXO1A only when phosphorylated at serine 256
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the sequence around phosphorylation site of serine 256 (

    A-A-S(p)-M-D

    ) of Human FOXO1A (NP_002006.2).

  • Positive control
    • MCF7 cells, 293 cell extracts, Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 78-82 kDa (predicted molecular weight: 69 kDa). Incubate membrane with diluted antibody in 5% non fatmilk, 1X TBS, 0.1% Tween 20 at 4 C, with gentle shaking, overnight.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKH 1 antibody
    • FKH1 antibody
    • FKHR antibody
    • Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
    • Forkhead box O1 antibody
    • Forkhead box protein O1 antibody
    • Forkhead box protein O1A antibody
    • Forkhead in rhabdomyosarcoma antibody
    • Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
    • FoxO transcription factor antibody
    • foxo1 antibody
    • FOXO1_HUMAN antibody
    • FOXO1A antibody
    • OTTHUMP00000018301 antibody
    see all

Images

  •  Immunofluorescent analysis of formalin-fixed, paraffin-embedded human breast tissue with Anti-FOXO1A at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.

  • All lanes : Anti-FOXO1A (phospho S256) antibody (ab131339) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts treated with serum

    Predicted band size: 69 kDa

  • Immunofluorescence analysis of methanol-fixed MCF7 cells using ab131339 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131339 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.

References

This product has been referenced in:
  • Feng YL  et al. Resveratrol prevents osteoporosis by upregulating FoxO1 transcriptional activity. Int J Mol Med 41:202-212 (2018). Read more (PubMed: 29115382) »
  • Pantaleão LC  et al. Prolonged fasting elicits increased hepatic triglyceride accumulation in rats born to dexamethasone-treated mothers. Sci Rep 7:10367 (2017). Read more (PubMed: 28871187) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblast and stem cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
Human fibroblast and stem cell
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 06 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Retrieval Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Brain Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 26 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.2% Triton X100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Muscle)
Gel Running Conditions
Reduced Denaturing (3-8%)
Loading amount
30 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 04 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cell (muscle)
Specification
muscle
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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