Anti-FOXO3A antibody (ab17026)
Key features and details
- Goat polyclonal to FOXO3A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-FOXO3A antibody
See all FOXO3A primary antibodies -
Description
Goat polyclonal to FOXO3A -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
GAKQASSQSWVPG
, corresponding to amino acids 661-673 of Human FOXO3A. -
Positive control
- Recombinant Human FOXO3A protein (ab114191) can be used as a positive control in WB. Human heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsIn the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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Database links
- Entrez Gene: 2309 Human
- Omim: 602681 Human
- SwissProt: O43524 Human
- Unigene: 220950 Human
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Alternative names
- AF6q21 antibody
- AF6q21 protein antibody
- DKFZp781A0677 antibody
see all
Images
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All lanes : Anti-FOXO3A antibody (ab17026) at 0.3 µg/ml
Lane 1 : Human Heart tissue lysate
Lane 2 : Human Heart tissue lysate with immunising peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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All lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution
All lanes : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 72 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible non-specific binding)
Exposure time: 5 minutesBlocked for 1 hour at 22°C.
Incubated with the primary antibody for 20 hours at 4°C.
Datasheets and documents
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Datasheet download
References (17)
ab17026 has been referenced in 17 publications.
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Yeh CC et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep 25:N/A (2022). PubMed: 34913071
- Du Y & Ning JZ MiR-182 Promotes Ischemia/Reperfusion-Induced Acute Kidney Injury in Rat by Targeting FoxO3. Urol Int 105:687-696 (2021). PubMed: 33965964
- Li Z et al. Kcnh2 mediates FAK/AKT-FOXO3A pathway to attenuate sepsis-induced cardiac dysfunction. Cell Prolif 54:e12962 (2021). PubMed: 33263944
- Lu X et al. Modulation of HOXA9 after skeletal muscle denervation and reinnervation. Am J Physiol Cell Physiol 318:C1154-C1165 (2020). PubMed: 32233950