Overview

  • Product name
  • Description
    Goat polyclonal to FOXO3A
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GAKQASSQSWVPG

    , corresponding to amino acids 661-673 of Human FOXO3A.

  • Positive control
    • Recombinant Human FOXO3A protein (ab114191) can be used as a positive control in WB. Human brain and heart

Properties

Applications

Our Abpromise guarantee covers the use of ab17026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human FOXO3A peptide (ab45581).
ELISA 1/32000.

Target

  • Function
    Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF6q21 antibody
    • AF6q21 protein antibody
    • DKFZp781A0677 antibody
    • FKHR2 antibody
    • FKHRL 1 antibody
    • FKHRL1 antibody
    • FKHRL1P2 antibody
    • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead box O3 antibody
    • Forkhead box O3A antibody
    • Forkhead box protein O3 antibody
    • Forkhead box protein O3A antibody
    • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
    • Forkhead homolog (rhabdomyosarcoma) like 1 antibody
    • Forkhead in rhabdomyosarcoma like 1 antibody
    • Forkhead in rhabdomyosarcoma-like 1 antibody
    • FOX O3A antibody
    • FOXO2 antibody
    • foxo3 antibody
    • FOXO3_HUMAN antibody
    • FOXO3A antibody
    • MGC12739 antibody
    • MGC31925 antibody
    see all

Images

  • Anti-FOXO3A antibody (ab17026) at 0.1 µg/ml + Human brain lysates at 35 µg

    Predicted band size: 72 kDa



    ab17026 at 0.5ug/ml on human brain lysate (35ug per lane).
  • All lanes : Anti-FOXO3A antibody (ab17026) at 1/1000 dilution

    All lanes : Human hepatic epithelial-like cell line (AKN-1) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Alkaline Phosphatase conjugated-mouse anti-goat IgG monoclonal at 1/15000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 72 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa (possible non-specific binding)


    Exposure time: 5 minutes


    Blocked for 1 hour at 22°C.

    Incubated with the primary antibody for 20 hours at 4°C.

    See Abreview

References

This product has been referenced in:
  • Tarpey MD  et al. Skeletal muscle autophagy and mitophagy in endurance-trained runners before and after a high-fat meal. Mol Metab 6:1597-1609 (2017). Read more (PubMed: 29097020) »
  • Liu G  et al. aMSH inhibits adipose inflammation via reducing FoxOs transcription and blocking Akt/JNK pathway in mice. Oncotarget 8:47642-47654 (2017). Read more (PubMed: 28514752) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatic epithelial-like cell line (AKN-1))
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
30 µg
Specification
human hepatic epithelial-like cell line (AKN-1)
Blocking step
I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 14 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
100000 cells
Specification
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCap (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCap (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 02 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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