Product nameAnti-FOXO3A antibody
See all FOXO3A primary antibodies
DescriptionRabbit polyclonal to FOXO3A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 625 and the C terminus (residue 673) of human FOXO3A. (Swiss-Prot O43524).
- Whole cell lysate from MCF7 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 71 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTranscriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
Involvement in diseaseNote=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsIn the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
Cellular localizationCytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
- Information by UniProt
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All lanes : Anti-FOXO3A antibody (ab70315) at 0.1 µg/ml
Lane 1 : HeLa cell Lysate
Lane 2 : 293T cell Lysate
Lane 3 : Jurkat cell Lysate
Lane 4 : Mouse TCMK-1 cell Lysate
Lane 5 : Mouse NIH3T3 cell Lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 3 minutes
Lane 1: MCF7 cell Lysate immunoprecipitated with ab70314 at 6 ug/mg of Lysate
Lane 2: MCF7 cell Lysate immunoprecipitated with ab70315 at 6 ug/mg of Lysate
Lane 3: MCF7 cell Lysate immunoprecipitated with Control IgG at 6 ug/mg of Lysate
Western Blot carried out with ab70315 at 1ug/ml.
Dectection by ECL for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling FOXO3A with ab70315 at 1/1000 (1µg/ml). Detection: DAB.
This product has been referenced in:
- Xu D et al. SIRT2 Inhibition Results in Meiotic Arrest, Mitochondrial Dysfunction, and Disturbance of Redox Homeostasis during Bovine Oocyte Maturation. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30889926) »
- Penolazzi L et al. MicroRNA-221 silencing attenuates the degenerated phenotype of intervertebral disc cells. Aging (Albany NY) 10:2001-2015 (2018). Read more (PubMed: 30130742) »