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Inquiry: trying to purchase p-FOXO3a (Ser253)antibody. There are several antibodies for FOXO3a (ab-47285, ab31109, ab154786). They detected different different size of FOXO3a-ser253 protein. Why?
Asked on Jun 09 2015
Thank you for contacting us.
The predicted MW of the protein is ˜ 75 kD, based on sequence alone.
However page 4 of paper attached showed, phosphorylation on serine 253 of FOXO3A/FKHRL1 could migrate the MW to ˜100KD for this protein.
Heather AllenAbcam Scientific Support
Answered on Jun 09 2015