For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Phospho specific peptide corresponding to residues surrounding Serine 253 of Human FOXO3A (UniProt O43524).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab154786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IHC-P||1/50 - 1/100.|
|Dot blot||Use at an assay dependent concentration.|
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"