Recombinant
RabMAb

Recombinant Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free (ab240127)

Overview

  • Product name

    Anti-FOXO3A (phospho S253) antibody [EPR1951(2)] - BSA and Azide free
    See all FOXO3A primary antibodies
  • Description

    Rabbit monoclonal [EPR1951(2)] to FOXO3A (phospho S253) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FOXO3A. The exact sequence is proprietary.

  • General notes

    Ab240127 is the carrier-free version of ab154786. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
    • Information by UniProt
    • Database links

    • Alternative names

      • AF6q21 antibody
      • AF6q21 protein antibody
      • DKFZp781A0677 antibody
      • FKHR2 antibody
      • FKHRL 1 antibody
      • FKHRL1 antibody
      • FKHRL1P2 antibody
      • Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
      • Forkhead box O3 antibody
      • Forkhead box O3A antibody
      • Forkhead box protein O3 antibody
      • Forkhead box protein O3A antibody
      • Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
      • Forkhead homolog (rhabdomyosarcoma) like 1 antibody
      • Forkhead in rhabdomyosarcoma like 1 antibody
      • Forkhead in rhabdomyosarcoma-like 1 antibody
      • FOX O3A antibody
      • FOXO2 antibody
      • foxo3 antibody
      • FOXO3_HUMAN antibody
      • FOXO3A antibody
      • MGC12739 antibody
      • MGC31925 antibody
      see all

    Images

    • Dot Blot analysis of Lane 1: FOXO3A (pS253) phospho peptide and Lane 2: FOXO3A non-phospho peptide with ab154786 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer and concentration. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human normal pancreas tissue labeling FOXO3A (phospho S253) with ab154786 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154786).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240127 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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