Overview

  • Product name
  • Description
    Rabbit polyclonal to FOXO4 / AFX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human FOXO4/ AFX aa 268-505 (internal sequence).
    Database link: P98177

  • Positive control
    • 293T; A431: H1299; HeLa; HepG2; MOLT4 and Raji cell lysates; HeLa cells; HBL435 xenograft.
  • General notes

    Previously labelled as FOXO4. 

Properties

Applications

Our Abpromise guarantee covers the use of ab154520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  • Tissue specificity
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
  • Involvement in disease
    Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 fork-head DNA-binding domain.
  • Post-translational
    modifications
    Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
    Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
    Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
  • Cellular localization
    Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFX antibody
    • AFX1 antibody
    • Afxh antibody
    • ALL1-fused gene from X chromosome antibody
    • Fork head domain transcription factor AFX1 antibody
    • Forkhead box O4 antibody
    • Forkhead box protein O4 antibody
    • FOXO 4 antibody
    • Foxo4 antibody
    • FOXO4_HUMAN antibody
    • MGC117660 antibody
    • MGC120490 antibody
    • Mixed lineage leukemia, translocated to, 7 antibody
    • MLLT7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
    • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
    • RGD1561201 antibody
    see all

Images

  • Anti-FOXO4 / AFX antibody (ab154520) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, labeling FOXO4 / AFX using ab154520 at 1/500 dilution.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling FOXO4 / AFX using ab154520 at 1/200 dilution. Right panel costained with Hoechst 33342.

References

ab154520 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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