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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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Anti-FOXO4/AFX antibody (ab245453)

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Western blot - Anti-FOXO4/AFX antibody (ab245453)

    Key features and details

    • Rabbit polyclonal to FOXO4/AFX
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant Human FOXO4/AFX protein (ab252397)

    View more associated products

    Overview

    • Product name

      Anti-FOXO4/AFX antibody
      See all FOXO4/AFX primary antibodies
    • Description

      Rabbit polyclonal to FOXO4/AFX
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human FOXO4/AFX aa 455-505. The exact sequence is proprietary. NP_005929.2
      Database link: P98177

    • Positive control

      • WB: HeLa whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab245453 was affinity purified using an epitope specific to FOXO4/AFX immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • FOXO

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
    • Recombinant Protein

      • Recombinant Human FOXO4/AFX protein (ab252397)

    Applications

    Our Abpromise guarantee covers the use of ab245453 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.

    Target

    • Function

      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • Involvement in disease

      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • Cellular localization

      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Target information above from: UniProt accession P98177 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4303 Human
      • Omim: 300033 Human
      • SwissProt: P98177 Human
      • Unigene: 584654 Human
      • Alternative names

        • AFX antibody
        • AFX1 antibody
        • Afxh antibody
        • ALL1-fused gene from X chromosome antibody
        • Fork head domain transcription factor AFX1 antibody
        • Forkhead box O4 antibody
        • Forkhead box protein O4 antibody
        • FOXO 4 antibody
        • Foxo4 antibody
        • FOXO4_HUMAN antibody
        • MGC117660 antibody
        • MGC120490 antibody
        • Mixed lineage leukemia, translocated to, 7 antibody
        • MLLT7 antibody
        • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
        • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
        • RGD1561201 antibody
        see all

      Images

      • Western blot - Anti-FOXO4/AFX antibody (ab245453)
        Western blot - Anti-FOXO4/AFX antibody (ab245453)
        All lanes : Anti-FOXO4/AFX antibody (ab245453) at 0.1 µg/ml

        Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
        Lane 2 : HeLa whole cell lysate at 15 µg
        Lane 3 : HeLa whole cell lysate at 5 µg

        Developed using the ECL technique.

        Predicted band size: 54 kDa


        Exposure time: 3 minutes

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab245453? Please let us know so that we can cite the reference in this datasheet.

      ab245453 has not yet been referenced specifically in any publications.

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