Overview

  • Product name

    Anti-FOXO4/AFX antibody [EPR5442]
    See all FOXO4/AFX primary antibodies
  • Description

    Rabbit monoclonal [EPR5442] to FOXO4/AFX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FOXO4/AFX aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, fetal heart, fetal muscle, fetal lung, and HuT-78 lysates. Permeabilized 293T cells.
  • General notes

    This antibody was developed as part of a collaboration between the University of Utrecht in the Netherlands and the lab of Tobias Dansen.

    Previously labelled as FOXO4. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • Involvement in disease

      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • Cellular localization

      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    Images

    • All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Fetal heart lysate
      Lane 3 : Fetal muscle lysate
      Lane 4 : Fetal lung lysate
      Lane 5 : HuT-78 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of FOXO4/AFX in permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells using ab128908 at 1/100 dilution (Red) compared to a non-specific negative control antibody (green).

    • All lanes : Anti-FOXO4/AFX antibody [EPR5442] (ab128908) at 1/1000 dilution

      Lane 1 : 293T transfected with FOXO4/AFX
      Lane 2 : 293T cell lysate

      Secondary
      Lane 1 : Goat-anti-rabbit HRP at 1/2000 dilution
      Lane 2 : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 63 kDa why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Liu H  et al. Inhibition of Brd4 alleviates renal ischemia/reperfusion injury-induced apoptosis and endoplasmic reticulum stress by blocking FoxO4-mediated oxidative stress. Redox Biol 24:101195 (2019). Read more (PubMed: 31004990) »
    • Zhang F  et al. Oncogenic RAS-induced CK1a drives nuclear FOXO proteolysis. Oncogene 37:363-376 (2018). WB . Read more (PubMed: 28945225) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Specification
    rat vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

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