Product nameAnti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)]
See all FOXO4/AFX primary antibodies
DescriptionRabbit monoclonal [EPR2315(2)] to FOXO4/AFX (phospho S262)
This antibody only detects FOXO4 / AFX phosphorylated at serine 262.
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human FOXO4/AFX.
- FOXO4/AFX transfected 293T cell lysate.
This antibody was developed as part of a collaboration between the University of Utrecht, Netherlands and the lab of Tobias Dansen.
Previously labelled as FOXO4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.92 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 53 kDa).|
FunctionTranscription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Involvement in diseaseNote=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsAcetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Cellular localizationCytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
- Information by UniProt
- AFX antibody
- AFX1 antibody
- Afxh antibody
All lanes : Anti-FOXO4/AFX (phospho S262) antibody [EPR2315(2)] (ab126594) at 1/10000 dilution
Lane 1 : Cell lysate from FOXO4 transfected 293T
Lane 2 : Cell lysate from FOXO4 transfected 293T treated with Alkaline Phosphatase
Lane 3 : Cell lysate from non-transfected 293T
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zhang F et al. Oncogenic RAS-induced CK1a drives nuclear FOXO proteolysis. Oncogene 37:363-376 (2018). WB . Read more (PubMed: 28945225) »
- Mandai S et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). Read more (PubMed: 29904119) »