Product nameAnti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)]
See all FOXO4/AFX primary antibodies
DescriptionRabbit monoclonal [EPR2314(2)] to FOXO4/AFX (phospho T32)
Only detects FOXO4 / AFX phosphorylated at Threonine 32.
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
corresponding to Human FOXO4/AFX.
- 293T transfected with HA-FOXO4/AFX
This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as FOXO4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (KD)KD = 2.51 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 53 kDa.|
FunctionTranscription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
Involvement in diseaseNote=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
modificationsAcetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Cellular localizationCytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
- Information by UniProt
- AFX antibody
- AFX1 antibody
- Afxh antibody
All lanes : Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : 293T transfected with HA-FOXO4/AFX cell lysates
Lane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates
Predicted band size: 53 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab128876 has not yet been referenced specifically in any publications.