Overview

  • Product name

    Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)]
    See all FOXO4/AFX primary antibodies
  • Description

    Rabbit monoclonal [EPR2314(2)] to FOXO4/AFX (phospho T32)
  • Host species

    Rabbit
  • Specificity

    Only detects FOXO4 / AFX phosphorylated at Threonine 32.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human FOXO4/AFX.

  • Positive control

    • 293T transfected with HA-FOXO4/AFX
  • General notes

    This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as FOXO4. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
    • Tissue specificity

      Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
    • Involvement in disease

      Note=A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.
    • Sequence similarities

      Contains 1 fork-head DNA-binding domain.
    • Post-translational
      modifications

      Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity.
      Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization.
      Monoubiquitinated; monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors; results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7; deubiquitination is induced by oxidative stress; enhances its interaction with USP7 and consequently, deubiquitination; increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
    • Cellular localization

      Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • AFX antibody
      • AFX1 antibody
      • Afxh antibody
      • ALL1-fused gene from X chromosome antibody
      • Fork head domain transcription factor AFX1 antibody
      • Forkhead box O4 antibody
      • Forkhead box protein O4 antibody
      • FOXO 4 antibody
      • Foxo4 antibody
      • FOXO4_HUMAN antibody
      • MGC117660 antibody
      • MGC120490 antibody
      • Mixed lineage leukemia, translocated to, 7 antibody
      • MLLT7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
      • Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
      • RGD1561201 antibody
      see all

    Images

    • All lanes : Anti-FOXO4/AFX (phospho T32) antibody [EPR2314(2)] (ab128876) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : 293T transfected with HA-FOXO4/AFX cell lysates
      Lane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates

      Predicted band size: 53 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128876 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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