RecombinantRabMAb

Recombinant Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)

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Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)
  • Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6863] to Foxo6 (phospho S184)
  • Suitable for: WB
  • Reacts with: Rat

Overview

  • Product name

    Anti-Foxo6 (phospho S184) antibody [EPR6863]
    See all Foxo6 primary antibodies

  • Description

    Rabbit monoclonal [EPR6863] to Foxo6 (phospho S184)

  • Host species

    Rabbit

  • Specificity

    ab154832 only detects Foxo6 phosphorylated at Serine 184.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide, corresponding to residues surrounding Serine 184 of Human Foxo6 (UniProt: A8MYZ6).

  • Positive control

    • Rat brain lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Relevance

      Murine Foxo6 is a member of the murine forkhead family of transcription factors. This family consists of over 30 members, the vast majority of which is important in embryonic development. These forkhead transcription factors may play a role in maintenance and survival of developing and adult neurons.

    • Cellular localization

      Cytoplasmic and Nuclear. When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors.

    • Database links

      • Alternative names

        • Forkhead box protein O6 antibody

      Images

      • Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)
        Western blot - Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)
        All lanes : Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832) at 1/1000 dilution

        Lane 1 : Rat brain lysate, untreated
        Lane 2 : Rat brain lysate, treated with AP

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 61 kDa

      • Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)
        Anti-Foxo6 (phospho S184) antibody [EPR6863] (ab154832)

      References (1)

      Publishing research using ab154832? Please let us know so that we can cite the reference in this datasheet.

      ab154832 has been referenced in 1 publication.

      • Calvert AE  et al. Cancer-Associated IDH1 Promotes Growth and Resistance to Targeted Therapies in the Absence of Mutation. Cell Rep 19:1858-1873 (2017). PubMed: 28564604

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