Product nameAnti-FOXP1 antibody
See all FOXP1 primary antibodies
DescriptionRabbit polyclonal to FOXP1
Tested applicationsSuitable for: IHC-FoFr, IP, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Dog, Xenopus laevis
- This antibody gave a positive signal in Jurkat nuclear lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||1/200 - 1/500. PubMed: 20364144|
|IP||Use at an assay dependent concentration.|
|WB||1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionTranscriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
Involvement in diseaseNote=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
DomainThe leucine-zipper is required for dimerization and transcriptional repression.
- Information by UniProt
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
Image courtesy of Human Protein Atlas
ab16645 staining FOXP1 in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab16645 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab16645 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab14404 staining FOXP1 in mouse pancreatic cancer primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1), permeabilized with Triton 0.025% in PBS and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 4 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
This product has been referenced in:
- Li H et al. Generality and opponency of rostromedial tegmental (RMTg) roles in valence processing. Elife 8:N/A (2019). Read more (PubMed: 30667358) »
- Nam H et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. Elife 8:N/A (2019). Read more (PubMed: 31305241) »