Anti-FOXP1 antibody (ab16645)
Key features and details
- Rabbit polyclonal to FOXP1
- Suitable for: IHC-FoFr, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FOXP1 antibody
See all FOXP1 primary antibodies -
Description
Rabbit polyclonal to FOXP1 -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab16645 are tested in IHC-P. Previous batches were additionally validated in IHC-FoFr and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab134055.
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Tested applications
Suitable for: IHC-FoFr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Chicken, Dog, Human, Xenopus laevis -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-p: Mouse brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (7) |
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
Notes |
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IHC-FoFr
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details). |
Target
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Function
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
Involvement in disease
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 416092 Chicken
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Entrez Gene: 496386 Xenopus laevis
- Entrez Gene: 779033 Xenopus laevis
- Omim: 605515 Human
see all -
Alternative names
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
Images
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Paraffin-embedded mouse brain tissue stained for FOXP1 using ab16645 in immunohistochemical analysis.
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All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity) -
ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (97)
ab16645 has been referenced in 97 publications.
- Limone F et al. Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cues. Cell Rep 42:111896 (2023). PubMed: 36596304
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- Wang W et al. The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development. Nat Commun 13:958 (2022). PubMed: 35177643
- Zhao YN et al. Whole-Brain Monosynaptic Afferents to Rostromedial Tegmental Nucleus Gamma-Aminobutyric Acid-Releasing Neurons in Mice. Front Neurosci 16:914300 (2022). PubMed: 35733933
- Li Z et al. Transcription factor Sp9 is a negative regulator of D1-type MSN development. Cell Death Discov 8:301 (2022). PubMed: 35773249