Anti-FOXP1 antibody - C-terminal (ab227788)
Key features and details
- Rabbit polyclonal to FOXP1 - C-terminal
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FOXP1 antibody - C-terminal
See all FOXP1 primary antibodies -
Description
Rabbit polyclonal to FOXP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil tissue. ICC/IF: MCF7 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
Involvement in disease
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 416092 Chicken
- Entrez Gene: 515903 Cow
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 100345241 Rabbit
- Entrez Gene: 297480 Rat
- Omim: 605515 Human
see all -
Alternative names
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling FOXP1 with ab227788 at 1/100 dilution (5 μg/ml), followed by ab150081 AlexaFluor®488 preadsorbed Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 μg/ml). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 AlexaFluor®488 preadsorbed Goat anti-Rabbit secondary antibody at 1/1000 dilution (2 μg/ml).
Confocal image showing nuclear staining in MCF7 cell lines. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP1 with ab227788 (1/100 dilution) in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227788 has not yet been referenced specifically in any publications.