Overview

  • Product name

    Anti-FOXP1 antibody [EPR4113]
    See all FOXP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4113] to FOXP1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FOXP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Daudi, MCF7, T47D, 2.4G2, RAW264.7 and Jurkat whole cell lysate (ab7899); mouse lung tissue lysates, Human tonsil tissue, Human diffuse large B-cell lymphoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50-95 kDa (predicted molecular weight: 75 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified format use at 1/250-1/500 dilution.

ICC 1/500 - 1/1000.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
    • Involvement in disease

      Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
      Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
    • Sequence similarities

      Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • Domain

      The leucine-zipper is required for dimerization and transcriptional repression.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 12CC4 antibody
      • FLJ23741 antibody
      • Fork head related protein like B antibody
      • Forkhead box P1 antibody
      • Forkhead box protein P1 antibody
      • FOX P1 antibody
      • FOXP 1 antibody
      • foxp1 antibody
      • FOXP1_HUMAN antibody
      • Glutamine rich factor 1 antibody
      • hFKH1B antibody
      • HSPC215 antibody
      • MGC12942 antibody
      • MGC88572 antibody
      • MGC99551 antibody
      • QRF 1 antibody
      • QRF1 antibody
      see all

    Images

    • Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/5000 dilution (Purified) + 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 75 kDa
      Observed band size: 50,70,95 kDa
      why is the actual band size different from the predicted?



      The multiple bands are consistent with what have been described in the literature PMID: 12692134.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human diffuse large B-cell lymphoma tissue sections labeling FOXP1 with purified ab134055 at 1/1000 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/1000 dilution (Purified)

      Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
      Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
      Lane 3 : Mouse lung cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 75 kDa
      Observed band size: 50,70,95 kDa why is the actual band size different from the predicted?



      The multiple bands are consistent with what have been described in the literature PMID: 12692134.

    • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FOXP1 with purified ab134055 at 1/500 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134055 (unpurified) showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab134055 (unpurified) showing +ve staining.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling FOXP1 with ab134055 (unpurified) at 1/250 dilution.

    • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab134055 (unpurified) showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134055 (unpurified) showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab134055 (unpurified) showing +ve staining.

    • Flow Cytometry analysis of Daudi (human Burkitt's lymphoma ) cells labeling FOXP1 with purified ab134055 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab134055 (unpurified) showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab134055 (unpurified) showing -ve staining.

    References

    This product has been referenced in:

    • Rodenas-Cuadrado PM  et al. Mapping the distribution of language related genes FoxP1, FoxP2, and CntnaP2 in the brains of vocal learning bat species. J Comp Neurol 526:1235-1266 (2018). IHC-P . Read more (PubMed: 29297931) »
    • Louis Sam Titus ASC  et al. Reduced Expression of Foxp1 as a Contributing Factor in Huntington's Disease. J Neurosci 37:6575-6587 (2017). Read more (PubMed: 28550168) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your inquiry.
    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.
    We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.
    Should you have any further questions, please do not hesitate to contact us.

    Read More

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