Overview

  • Product name

    Anti-FOXP1 antibody [EPR4113] (HRP)
    See all FOXP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4113] to FOXP1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human FOXP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 75 kDa).

Target

  • Function

    Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
  • Involvement in disease

    Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
    Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Domain

    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12CC4 antibody
    • FLJ23741 antibody
    • Fork head related protein like B antibody
    • Forkhead box P1 antibody
    • Forkhead box protein P1 antibody
    • FOX P1 antibody
    • FOXP 1 antibody
    • foxp1 antibody
    • FOXP1_HUMAN antibody
    • Glutamine rich factor 1 antibody
    • hFKH1B antibody
    • HSPC215 antibody
    • MGC12942 antibody
    • MGC88572 antibody
    • MGC99551 antibody
    • QRF 1 antibody
    • QRF1 antibody
    see all

Images

  • Anti-FOXP1 antibody [EPR4113] (HRP) (ab196978) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196978 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

This product has been referenced in:

  • Zhou W & Li R microRNA-605 inhibits the oncogenicity of non-small-cell lung cancer by directly targeting Forkhead Box P1. Onco Targets Ther 12:3765-3777 (2019). Read more (PubMed: 31190877) »
See 1 Publication for this product

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