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    foxp1-antibody-jc12-ab32010.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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Anti-FOXP1 antibody [JC12] (ab32010)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (33)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
  • Western blot - Anti-FOXP1 antibody [JC12] (ab32010)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
  • Western blot - Anti-FOXP1 antibody [JC12] (ab32010)

Key features and details

  • Mouse monoclonal [JC12] to FOXP1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2a

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Primary
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Anti-PAX5 antibody [EPR3730(2)] (ab109443)
Protein
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Recombinant Human FOXP1 protein (ab132166)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-FOXP1 antibody [JC12]
    See all FOXP1 primary antibodies
  • Description

    Mouse monoclonal [JC12] to FOXP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Zebra finch
  • Immunogen

    Full length native protein (purified) corresponding to Mouse FOXP1.

  • Positive control

    • WB: BCBL1 human cell line or Mutul human cell line. IHC-P: Human Hodgkin's lymphoma and human colon tissues.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 6.97% L-Arginine, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JC12
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Anti-FOXP1 antibody [JC12] - BSA and Azide free (ab264529)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human FOXP1 protein (ab132166)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (3)
1/500. Predicted molecular weight: 75 kDa.
Notes
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500. Predicted molecular weight: 75 kDa.

Target

  • Function

    Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
  • Involvement in disease

    Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
    Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Domain

    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9H334 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27086 Human
    • Entrez Gene: 108655 Mouse
    • Omim: 605515 Human
    • SwissProt: Q9H334 Human
    • SwissProt: P58462 Mouse
    • Unigene: 431498 Human
    • Unigene: 234965 Mouse
    • Unigene: 485266 Mouse
    • Alternative names

      • 12CC4 antibody
      • FLJ23741 antibody
      • Fork head related protein like B antibody
      • Forkhead box P1 antibody
      • Forkhead box protein P1 antibody
      • FOX P1 antibody
      • FOXP 1 antibody
      • foxp1 antibody
      • FOXP1_HUMAN antibody
      • Glutamine rich factor 1 antibody
      • hFKH1B antibody
      • HSPC215 antibody
      • MGC12942 antibody
      • MGC88572 antibody
      • MGC99551 antibody
      • QRF 1 antibody
      • QRF1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)

      IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

      The inset negative control image is taken from an identical assay without primary antibody.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

       

       

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-FOXP1 antibody [JC12] (ab32010)
      Western blot - Anti-FOXP1 antibody [JC12] (ab32010)
      All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml

      Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution

      Predicted band size: 75 kDa
      Observed band size: 100,80 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)

      IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 

      The inset negative control image is taken from an identical assay without primary antibody.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

       

       

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-FOXP1 antibody [JC12] (ab32010)
      Western blot - Anti-FOXP1 antibody [JC12] (ab32010)
      All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution

      Lane 1 : BCBL1 human cell line
      Lane 2 : Mutul human cell line

      Predicted band size: 75 kDa
      Observed band size: 60,65,80 kDa why is the actual band size different from the predicted?



      This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.

      Image kindly provided by H. Hu.

    Protocols

    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (33)

    Publishing research using ab32010? Please let us know so that we can cite the reference in this datasheet.

    ab32010 has been referenced in 33 publications.

    • Neyroud D  et al. FoxP1 is a transcriptional repressor associated with cancer cachexia that induces skeletal muscle wasting and weakness. J Cachexia Sarcopenia Muscle 12:421-442 (2021). PubMed: 33527776
    • Garcia-Oscos F  et al. Autism-linked gene FoxP1 selectively regulates the cultural transmission of learned vocalizations. Sci Adv 7:N/A (2021). PubMed: 33536209
    • Anderson AG  et al. Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development. Cell Rep 30:3051-3066.e7 (2020). PubMed: 32130906
    • Fjodorova M  et al. A role for TGFß signalling in medium spiny neuron differentiation of human pluripotent stem cells. Neuronal Signal 4:NS20200004 (2020). PubMed: 32714602
    • Ko YCK  et al. Gene Expression Profiling of Head and Neck Tumors Identifies FOXP1 and SOX10 Expression as Useful for Distinguishing Ameloblastoma From Basaloid Salivary Gland Tumors. Am J Surg Pathol 44:665-672 (2020). PubMed: 31895100
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-FOXP1 antibody [JC12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Thymus, MCF7 nuclear extract and cytosolic and nuc)
    Gel Running Conditions
    Reduced Denaturing (4-20% TGX gel)
    Loading amount
    30 µg
    Specification
    Thymus, MCF7 nuclear extract and cytosolic and nuc
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 19 2018

    Flow Cytometry abreview for Anti-FOXP1 antibody [JC12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Western blot abreview for Anti-FOXP1 [JC12] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (5% Tris-Glycine)
    Sample
    Zebra finch Cell lysate - whole cell (Hek overexpressing zebra finch FoxP1)
    Specification
    Hek overexpressing zebra finch FoxP1
    Blocking step
    Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 14 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP1 antibody [JC12]

    Below Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Specification
    brain tissue
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    Yes - PBS-T
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 12 2011

    Western blot abreview for Anti-FOXP1 antibody [JC12]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Loading amount
    60 µg
    Specification
    293T
    Treatment
    transfected with EGFP FOXP 1
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 07 2010

    Question

    No bands in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2011

    Answer

    Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in your blots. I look forward to hearing from you, and let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Nov 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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