Anti-FOXP1 antibody [JC12] (ab32010)
Overview
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Product name
Anti-FOXP1 antibody [JC12]
See all FOXP1 primary antibodies -
Description
Mouse monoclonal [JC12] to FOXP1 -
Host species
Mouse -
Specificity
This antibody does not recognise closely related molecules FOXP2, FOXP3 or FOXP4. -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Zebra finch -
Immunogen
Full length native protein (purified) corresponding to Mouse FOXP1.
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Positive control
- WB: BCBL1 human cell line or Mutul human cell line. IHC-P: Human Hodgkin's lymphoma and human colon tissues.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
JC12 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20145110 | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500. Predicted molecular weight: 75 kDa. |
Target
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Function
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
Involvement in disease
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Omim: 605515 Human
- SwissProt: Q9H334 Human
- SwissProt: P58462 Mouse
- Unigene: 431498 Human
- Unigene: 59368 Human
- Unigene: 234965 Mouse
see all -
Alternative names
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-274940-anti-foxp1-antibody-jc12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human colon*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-43628-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HEK293 Whole Cell Lysate Overexpressing FOXP1
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
Predicted band size: 75 kDa
Observed band size: 100,80 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-274942-anti-foxp1-antibody-jc12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [JC12] (ab32010)IHC image of FOXP1 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma (CD30+)*, showing positive staining in the mantle zone and negative staining in the Hodgkin's cells and germinal centre. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32010, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-FOXP1 antibody [JC12] (ab32010)](https://www.abcam.com/ps/products/32/ab32010/Images/ab32010-43629-anti-foxp1-antibody-jc12-western-blot.jpg)
Western blot - Anti-FOXP1 antibody [JC12] (ab32010)All lanes : Anti-FOXP1 antibody [JC12] (ab32010) at 1/500 dilution
Lane 1 : BCBL1 human cell line
Lane 2 : Mutul human cell line
Predicted band size: 75 kDa
Observed band size: 60,65,80 kDa why is the actual band size different from the predicted?
This antibody recognizes a long form and two short forms of human FOXP1 as described in Blood. 2008. Mar 1; 111(5): 2816-24.
Image kindly provided by H. Hu.
References
ab32010 has been referenced in 25 publications.
- Harrison DJ et al. The Effect of Tissue Preparation and Donor Age on Striatal Graft Morphology in the Mouse. Cell Transplant 27:230-244 (2018). PubMed: 29637815
- Kaneyama T & Shirasaki R Post-crossing segment of dI1 commissural axons forms collateral branches to motor neurons in the developing spinal cord. J Comp Neurol 526:1943-1961 (2018). PubMed: 29752714
- Sun X et al. STAT3 promotes tumour progression in glioma by inducing FOXP1 transcription. J Cell Mol Med 22:5629-5638 (2018). PubMed: 30134017
- Mikhailova A et al. Unbiased Quantification of Subplate Neuron Loss following Neonatal Hypoxia-Ischemia in a Rat Model. Dev Neurosci 39:171-181 (2017). PubMed: 28434006
- Usui N et al. Foxp1 regulation of neonatal vocalizations via cortical development. Genes Dev 31:2039-2055 (2017). PubMed: 29138280
