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  1. Link

    foxp2-antibody-ab16046.pdf

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Anti-FOXP2 antibody (ab16046)

  • Datasheet
  • SDS
Reviews (20)Q&A (6)References (136)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)
  • Western blot - Anti-FOXP2 antibody (ab16046)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
  • Western blot - Anti-FOXP2 antibody (ab16046)

Key features and details

  • Rabbit polyclonal to FOXP2
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FOXP2 antibody
    See all FOXP2 primary antibodies
  • Description

    Rabbit polyclonal to FOXP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human FOXP2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16278)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000. (see abreview)
ICC/IF (2)
Use at an assay dependent concentration.
IHC-P (4)
Use at an assay dependent concentration.
Notes
WB
1/1000. (see abreview)
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • Tissue specificity

    Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • Involvement in disease

    Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Developmental stage

    Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • Domain

    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O15409 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 93986 Human
    • Entrez Gene: 114142 Mouse
    • Entrez Gene: 500037 Rat
    • Omim: 605317 Human
    • SwissProt: O15409 Human
    • SwissProt: P58463 Mouse
    • SwissProt: P0CF24 Rat
    • Unigene: 282787 Human
    • Unigene: 332919 Mouse
    • Unigene: 225249 Rat
    see all
  • Alternative names

    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)

    IHC image of FOXP2 staining in mouse e17 foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16046, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)

    ICC/IF image of ab16046 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-FOXP2 antibody (ab16046)
    Western blot - Anti-FOXP2 antibody (ab16046)This image is courtesy of an anonymous Abreview

    ab16046 at 1/1000 detecting FOXP2 from human 293T cell lysate (whole cell) (60ug/lane) by Western Blot. An HRP conjugated goat anti-rabbit IgG was used as the secondary and ECL was used as the detection method (1 minute exposure).

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)This image is courtesy of an abreview submitted by Jeremy Dasen.

    Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)Image courtesy of Human Protein Atlas

    Image courtesy of Human Protein Atlas

    ab16046 staining FOXP2 in human testis. Paraffin embedded human testis tissue was incubated with ab16046 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab16046 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Western blot - Anti-FOXP2 antibody (ab16046)
    Western blot - Anti-FOXP2 antibody (ab16046)
    Anti-FOXP2 antibody (ab16046) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size: 90 kDa why is the actual band size different from the predicted?
    Additional bands at: 56 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (136)

Publishing research using ab16046? Please let us know so that we can cite the reference in this datasheet.

ab16046 has been referenced in 136 publications.

  • Lowenstein ED  et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268
  • Deng Y  et al. Progression of basal ganglia pathology in heterozygous Q175 knock-in Huntington's disease mice. J Comp Neurol 529:1327-1371 (2021). PubMed: 32869871
  • Huisman C  et al. The histone H3-lysine 4-methyltransferase Mll4 regulates the development of growth hormone-releasing hormone-producing neurons in the mouse hypothalamus. Nat Commun 12:256 (2021). PubMed: 33431871
  • Lau EO  et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife 10:N/A (2021). PubMed: 33899739
  • Frew J & Nygaard HB Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice. Acta Neuropathol Commun 9:57 (2021). PubMed: 33795008
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 26 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (brain)
Permeabilization
Yes - 0.3% Triton-X
Specification
brain
Blocking step
Serum as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Sarah Bottjer

Verified customer

Submitted Sep 23 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Cervical cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA with pH8
Permeabilization
Yes - Triton X 100
Specification
Cervical cancer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 06 2021

Immunohistochemistry (Frozen sections) abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (spinal cord)
Specification
spinal cord
Blocking step
Gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

IHC - Wholemount abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Embryo (spinal cord)
Specification
spinal cord
Read More

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

IHC - Wholemount abreview for Anti-FOXP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 whole embryo)
Specification
E12.5 whole embryo
Read More

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton X-100
Specification
Brain
Fixative
Paraformaldehyde
Read More

Marissa Co

Verified customer

Submitted Dec 28 2017

Western blot abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris Gel)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Mar 27 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP2 antibody

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole brain slices)
Antigen retrieval step
None
Permeabilization
Yes - 10% Tween-20 diluted to working concentration of 0.01% in TBS
Specification
Whole brain slices
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 27 2017

Western blot abreview for Anti-FOXP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebra finch Recombinant protein (Hek overexpressing zebra finch FoxP2)
Loading amount
10 µg
Specification
Hek overexpressing zebra finch FoxP2
Gel Running Conditions
Reduced Denaturing (5% Tris-Glycine)
Blocking step
Roti Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted May 15 2013

Question

Inquiry: Western blot - mouse brain lysate - 40 ug/lane -1:1000 antibody dilution -HRP conjugated secondary I had 4 brain areas: cortex, cerebellum, hippocampus, and midbrain. In the cortex and hippocampus, i saw a very dark band around 150 kDa that overshadowed the much smaller foxp2 band. I was wondering if this has been seen before, and what that particular band is? Thanks.

Read More

Abcam community

Verified customer

Asked on Jul 17 2012

Answer

Thank you for your enquiry.

Perhaps it would be helpful if you could forward the western blot image along as an attachement?

Thanks for the additional information!

Read More

Abcam Scientific Support

Answered on Jul 17 2012

1-10 of 26 Abreviews or Q&A

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