Overview

  • Product name

  • Description

    Goat polyclonal to FOXP2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.1) and isoform III (NP_683697.1 and NP_683698.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    C-DEVEYQKRRSQKIT

    , corresponding to internal sequence amino acids 576-589 of Human FOXP2

  • Positive control

    • WB: Human Brain (Cerebellum) and rat brain lysate IHC-P: Human prostate and placenta tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

Target

  • Function

    Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • Tissue specificity

    Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • Involvement in disease

    Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Developmental stage

    Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • Domain

    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

Images

  • Anti-FOXP2 antibody (ab58599) at 0.3 µg/ml + Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer)

    Predicted band size: 80 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 29 kDa. We are unsure as to the identity of these extra bands.



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemical analysis of human placenta tissue staining FOXP2 with ab58599 at 2.5µg/ml. Heat-mediated antigen retrieval with citrate buffer (ph 6.0) was performed. Antibody was revealed with AP-staining.

  • ab58599 at 2.5 µg/ml staining FOXP2 in Human prostate by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat-mediated antigen retrieval with citrate buffer (ph 6.0) was performed. Antibody was revealed with AP-staining.

References

This product has been referenced in:

  • Li H  et al. SNHG1 promotes malignant biological behaviors of glioma cells via microRNA-154-5p/miR-376b-3p- FOXP2- KDM5B participating positive feedback loop. J Exp Clin Cancer Res 38:59 (2019). Read more (PubMed: 30728054) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab58599.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up