Overview

  • Product name
    Anti-FOXP2 antibody - C-terminal
    See all FOXP2 primary antibodies
  • Description
    Goat polyclonal to FOXP2 - C-terminal
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognise all three reported isoforms (NP_055306.1; NP_683696.2; NP_683697.1).
  • Tested applications
    Suitable for: WB, IHC-P, IHC - Wholemount, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebra finch
    Predicted to work with: Rat, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human FOXP2 aa 703-715 (C terminal) (Cysteine residue).
    Sequence:

    C-REIEEEPLSEDLE


    Database link: O15409
    (Peptide available as ab22800)

  • Positive control
    • WB: Whole tissue lysate from human cerebellum. IHC-Fr: e12.5 mouse embryonic spinal cord tissue. IHC-P: Human testis tissue. ICC/IF: Mouse e12.5 thoracic spinal cord cells. IHC-wholemount: Mouse brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab1307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa).Can be blocked with Human FOXP2 peptide (ab22800).
IHC-P Use at an assay dependent concentration. PubMed: 16901978
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23967072
IHC-Fr Use at an assay dependent concentration. PubMed: 20484636

Target

  • Function
    Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • Tissue specificity
    Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • Involvement in disease
    Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Developmental stage
    Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • Domain
    The leucine-zipper is required for dimerization and transcriptional repression.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

Images

  • Paraffin-embedded human testis tissue stained for FOXP2 with ab1307 at 5 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6. AP staining.

  • ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.

    See Abreview

  • Anti-FOXP2 antibody - C-terminal (ab1307) at 0.5 µg/ml + Whole tissue lysate from human cerebellum (35µg protein in RIPA buffer)

    Predicted band size: 79.9 kDa

  • Mouse brain tissue stained for FOXP2 with ab1307 (1/1000 dilution) in immunohistochemical analysis.

    Brain explant taken from E15 embryo and cultured for 1 month. Fixed for 4 hours in 4% PFA, permeabilised with PBS-TritonX 100 (0,25%). FOXP2 antibody (ab1307) was used at 1/1000 and a monoclonal mouse antibody was used to visualize CaBP. Secondary antibody incubated at RT for 2 hours.
    Image shows and overlay of FoxP2-positive deep cerebellar nuclei neurons (red) and CaBP-positive Purkinje cells (green).

    See Abreview

  • Mouse e12.5 thoracic spinal cord stained for FOXP2 (green) using ab1307 (1/2000 dilution) in ICC/IF.

References

This product has been referenced in:
  • Burkett ZD  et al. FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch. Elife 7:N/A (2018). Read more (PubMed: 29360038) »
  • Fong WL  et al. Differential and Overlapping Pattern of Foxp1 and Foxp2 Expression in the Striatum of Adult Mouse Brain. Neuroscience 388:214-223 (2018). Read more (PubMed: 30031127) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Xenopus laevis Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton X
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 21 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain

Ms. Lea Hachigian

Verified customer

Submitted Jul 23 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Mouse Cell lysate - whole cell (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Brain explant)
Specification
Brain explant

Abcam user community

Verified customer

Submitted Sep 28 2012

Question
Answer

That's my pleasure.

If you have any further questions in the future please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of https://www.abcam.com/ab16046.The order number is *******.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you forcontacting us. I am sorry to hear that this antibody is not providing satisfactory results.

Theuse of this antibody inIHC-Frwasfirst describedby one of our customersand his result andprotocol can be foundon the Abreview: https://www.abcam.com/index.html?datasheet=1307&tab=abreviews&intabreviewid=20555.

Shouldthesuggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (e12.5 mouse embryonic spinal cord)
Specification
e12.5 mouse embryonic spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Dr. Frederic Clotman

Verified customer

Submitted Sep 17 2010

Answer

Thank you for your email. As soon as we receive new information regarding our products, we update the online datasheets. So keep an eye on the datasheets for more information. Also, we're always adding new products, and I do know that we will be adding additional FOXP antibodies to our catalogue in the future. Please let me know if you have any questions in the future!

Read More

Answer

Thank you for your email. I have checked with the originator of this antibody who has provided me with the following information regarding your question. It seems that since the design of Ab1307, more members of the FOXP family have been identified, including other isoforms of FOXP2. We agree that there remains a possibility of cross-reaction with FOXP1 and FOXP4. Here is some data with the C-terminus in the third column and molecular weight in the fourth column: FOXP2 isoform 1 reieeeplsedle 79919 MW NP_055306 isoform 2 reieeeplsedle 82551 MW NP_683696 isoform 3 reieeeplsedle 70104 MW NP_683698 FOXP1 rdyedepvnedme 75317 MW NP_116071 FOXP4 rdleeelpgeels 72216 MW NP_612466 We haven't tested specifically for cross-reaction and can only be confident about our own data - which we have added to the datasheet: The WB in the data sheet of Ab1307 on mouse brain lysate indicate a clear ~75kDa band, and a weaker band of slightly higher molecular weight. Ab1307 stains acetone fixed cytospin preparation of Cos cells transfected with full length human FOX P2 cDNA by immunocytochemistry. Unfortunately we have no plans for specific tests of cross-reactivity and don’t have access to purified FOXP1, 2 or 4, nor have we access to knock-out tissue or the relevant peptides. We would be very grateful for any feedback from users on this point. With regard to the refund policy, we can only offer refunds if the product does not perform as described on this datasheet. However, Abcam does provide rewards for feedback from customers who have used our products. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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1-10 of 13 Abreviews or Q&A

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