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Our Abpromise guarantee covers the use of ab1307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa).Can be blocked with Human FOXP2 peptide (ab22800).|
|IHC-P||Use at an assay dependent concentration. PubMed: 16901978|
|IHC - Wholemount||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23967072|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20484636|
Paraffin-embedded human testis tissue stained for FOXP2 with ab1307 at 5 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6. AP staining.
ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.
Mouse brain tissue stained for FOXP2 with ab1307 (1/1000 dilution) in immunohistochemical analysis.
Brain explant taken from E15 embryo and cultured for 1 month. Fixed for 4 hours in 4% PFA, permeabilised with PBS-TritonX 100 (0,25%). FOXP2 antibody (ab1307) was used at 1/1000 and a monoclonal mouse antibody was used to visualize CaBP. Secondary antibody incubated at RT for 2 hours.
Image shows and overlay of FoxP2-positive deep cerebellar nuclei neurons (red) and CaBP-positive Purkinje cells (green).
Mouse e12.5 thoracic spinal cord stained for FOXP2 (green) using ab1307 (1/2000 dilution) in ICC/IF.
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