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    foxp3-antibody-221d-ab253297.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Recombinant

Recombinant Anti-FOXP3 antibody [221D] (ab253297)

  • Datasheet
  • SDS
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Western blot - Anti-FOXP3 antibody [221D] (ab253297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
  • Anti-FOXP3 antibody [221D] (ab253297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [221D] to FOXP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-FOXP3 antibody [221D]
    See all FOXP3 primary antibodies
  • Description

    Mouse monoclonal [221D] to FOXP3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IHC-Fr
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate. IHC-P: Human stomach carcinoma, tonsil and colon tissue; Mouse spleen and thymus tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    221D
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-FOXP3 antibody [221D] - BSA and Azide free (ab255942)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab253297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1.514 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use a concentration of 1.514 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 47 kDa).
Notes
IHC-P
Use a concentration of 1.514 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use a concentration of 1.514 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 47 kDa).
Application notes
Is unsuitable for IHC-Fr.

Target

  • Function

    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50943 Human
    • Entrez Gene: 20371 Mouse
    • Omim: 300292 Human
    • SwissProt: Q9BZS1 Human
    • SwissProt: Q99JB6 Mouse
    • Unigene: 247700 Human
    • Unigene: 182291 Mouse
    • Alternative names

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Images

    • Western blot - Anti-FOXP3 antibody [221D] (ab253297)
      Western blot - Anti-FOXP3 antibody [221D] (ab253297)
      All lanes : Anti-FOXP3 antibody [221D] (ab253297) at 1.514 µg/ml

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-myc tag, whole cell lysate
      Lane 2 : HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 47 kDa
      Observed band size: 73 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      The molecular weight observed was in consistant with the literature (PMID: 17586580).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the human tonsil. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human colon. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

      Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human stomach carcinoma. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse spleen tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

      Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse thymus tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-FOXP3 antibody [221D] (ab253297)
      Anti-FOXP3 antibody [221D] (ab253297)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab253297? Please let us know so that we can cite the reference in this datasheet.

    ab253297 has been referenced in 2 publications.

    • Roncador G  et al. Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level. Eur J Immunol 35:1681-91 (2005). PubMed: 15902688
    • Roncador G  et al. FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma. Leukemia 19:2247-53 (2005). PubMed: 16193085

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