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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Cynomolgus Monkey Tissue sections (Spleen (Cyno-Monkey))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
Other product details
Non-Abcam antibody was used: Donkey anti-mouse secondary antibody
Host species: Donkey
Antigen Retrieval used EDTA, pH9.0 is very import for this antibody.
Citric buffer pH 6.0 used for AR didn't work for this antibody.
Submitted Jul 18 2012