Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [236A/E7]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Spleen (Cyno-Monkey))
Specification
Spleen (Cyno-Monkey)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C

Other product details

Dilution
1/100
Incubation time
30 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/2000

Additional data

Additional Notes
Antigen Retrieval used EDTA, pH9.0 is very import for this antibody.
Citric buffer pH 6.0 used for AR didn't work for this antibody.

Jing Ma

Verified customer

Submitted Jul 18 2012

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