Immunocytochemistry/ Immunofluorescence abreview for Anti-FOXP3 antibody [236A/E7]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human head & neck squamous cell carcinoma)
Specification
Human head & neck squamous cell carcinoma
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%) for 5 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA (5%) in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

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Submitted Jan 28 2013

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