Western blot abreview for Anti-FOXP3 antibody [236A/E7]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Loading amount
30 µg
Treatment
250 μg/kg LPS for 1 week
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: m-IgGk BP-HRP
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
13 second(s)
Bands
Specific: 50 kDa

Additional data

Abcam response
Thank you for submitting your valuable results with us. It seems the signal intensity is very high for your blot with signs of over-exposure. To improve the visualization, you can consider to try blocking with 5% skim milk, use a lower primary antibody working concentration and lower loading quantity.

Ms. Ji Hye Han

Verified customer

Submitted Oct 22 2018

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