Anti-FOXP3 antibody [2A11G9] (ab36607)
Overview
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Product name
Anti-FOXP3 antibody [2A11G9]
See all FOXP3 primary antibodies -
Description
Mouse monoclonal [2A11G9] to FOXP3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt, ELISAmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Ni-NTA purified truncated recombinant Foxp3 expressed in E. Coli strain BL21 (DE3).
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Positive control
- HEK293 cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2A11G9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab36607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/1000. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa). | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ELISA | 1/10000. |
Application notes
Is unsuitable for IHC-Fr or IHC-P.
Target
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Function
Probable transcription factor. Plays a critical role in the control of immune response. -
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50943 Human
- Entrez Gene: 20371 Mouse
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- SwissProt: Q99JB6 Mouse
- Unigene: 247700 Human
- Unigene: 182291 Mouse
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Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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![Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)](https://www.abcam.com/ps/products/36/ab36607/Images/ab36607-48999-anti-foxp3-antibody-2a11g9-flow-cytometry.jpg)
Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)](https://www.abcam.com/ps/products/36/ab36607/Images/ab36607-49002-anti-foxp3-antibody-2a11g9-western-blot.gif)
Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)All lanes : Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution
Lane 1 : Human truncated Foxp3 recombinant protein
Lane 2 : HEK293 cell extract
Lysates/proteins at 2 µg/ml per lane.
Predicted band size: 47 kDa
Observed band size: ~50-53 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)](https://www.abcam.com/ps/products/36/ab36607/Images/ab36607-49000-anti-foxp3-antibody-2a11g9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.
Protocols
Datasheets and documents
References
ab36607 has been referenced in 3 publications.
- Ji H et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 64:573-82 (2014). PubMed: 24935938
- Imam S et al. Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes. Mol Vis 19:1259-67 (2013). ICC/IF ; Mouse . PubMed: 23805032
- Singh TP et al. 8-methoxypsoralen plus ultraviolet A therapy acts via inhibition of the IL-23/Th17 axis and induction of Foxp3+ regulatory T cells involving CTLA4 signaling in a psoriasis-like skin disorder. J Immunol 184:7257-67 (2010). PubMed: 20488788
