Anti-FOXP3 antibody [2A11G9] (ab36607)

Submit a review Q&A (1)References (5)
Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
  • Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)

Key features and details

  • Mouse monoclonal [2A11G9] to FOXP3
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-FOXP3 antibody [2A11G9]
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [2A11G9] to FOXP3

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Ni-NTA purified truncated recombinant Foxp3 expressed in E. Coli strain BL21 (DE3).

  • Positive control

    • HEK293 cell extract.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/200 - 1/1000.
WB
1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/200 - 1/1000.
WB
1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.

    • Target

    • Function

      Probable transcription factor. Plays a critical role in the control of immune response.

    • Involvement in disease

      Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

    • Sequence similarities

      Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • AIID antibody
        • DIETER antibody
        • Forkhead box P3 antibody
        • Forkhead box protein P3 antibody
        • FOXP3 antibody
        • FOXP3_HUMAN antibody
        • FOXP3delta7 antibody
        • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
        • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
        • IPEX antibody
        • JM2 antibody
        • MGC141961 antibody
        • MGC141963 antibody
        • OTTHUMP00000025832 antibody
        • OTTHUMP00000025833 antibody
        • OTTHUMP00000226737 antibody
        • PIDX antibody
        • Scurfin antibody
        • XPID antibody
        see all

      Images

      • Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
        Flow Cytometry - Anti-FOXP3 antibody [2A11G9] (ab36607)
        Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      • Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
        Western blot - Anti-FOXP3 antibody [2A11G9] (ab36607)
        All lanes : Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution

        Lane 1 : Human truncated Foxp3 recombinant protein
        Lane 2 : HEK293 cell extract

        Lysates/proteins at 2 µg/ml per lane.

        Predicted band size: 47 kDa
        Observed band size: ~50-53 kDa why is the actual band size different from the predicted?

      • Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)
        Immunocytochemistry/ Immunofluorescence - Anti-FOXP3 antibody [2A11G9] (ab36607)

        ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.

      References (5)

      Publishing research using ab36607? Please let us know so that we can cite the reference in this datasheet.

      ab36607 has been referenced in 5 publications.

      • Park TY  et al. Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases. Sci Rep 9:15559 (2019). PubMed: 31664129
      • Su H  et al. Mycobacterium tuberculosis PPE60 antigen drives Th1/Th17 responses via Toll-like receptor 2-dependent maturation of dendritic cells. J Biol Chem 293:10287-10302 (2018). PubMed: 29739853
      • Ji H  et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 64:573-82 (2014). PubMed: 24935938
      • Imam S  et al. Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes. Mol Vis 19:1259-67 (2013). ICC/IF ; Mouse . PubMed: 23805032
      • Singh TP  et al. 8-methoxypsoralen plus ultraviolet A therapy acts via inhibition of the IL-23/Th17 axis and induction of Foxp3+ regulatory T cells involving CTLA4 signaling in a psoriasis-like skin disorder. J Immunol 184:7257-67 (2010). PubMed: 20488788

      Customer reviews and Q&As

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      Question

      Product Anti-FOXP3 antibody [2A11G9] (ab36607) is a mouse monoclonal Ab, and we are working with mouse tissue sections – is this Ab suitable for IHC staining with mouse tissue?

      Can you recommend a product or protocol to achieve clean mouse-on-mouse staining in IHC?

      Read More
      Answer

      Unfortunately, ab36607 is not recommended for IHC applications (IHC-Fr or IHC-P).

      For Mouse-on-Mouse (MOM) staining with other mouse-host antibodies, we can recommend the following IHC detection kit: Mouse on Mouse Polymer IHC Kit (ab127055).

      Alternatively, our suggested MOM Staining Protocol can be found here: MOM Staining Protocol.

      Read More

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