Product nameAnti-FOXP3 antibody [2A11G9]
See all FOXP3 primary antibodies
DescriptionMouse monoclonal [2A11G9] to FOXP3
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, ELISAmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivityReacts with: Mouse, Human
Ni-NTA purified truncated recombinant Foxp3 expressed in E. Coli strain BL21 (DE3).
- HEK293 cell extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Light chain typekappa
Our Abpromise guarantee covers the use of ab36607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|WB||1/500 - 1/2000. Detects a band of approximately 50-53 kDa (predicted molecular weight: 47 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProbable transcription factor. Plays a critical role in the control of immune response.
Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
- Information by UniProt
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
All lanes : Anti-FOXP3 antibody [2A11G9] (ab36607) at 1/1000 dilution
Lane 1 : Human truncated Foxp3 recombinant protein
Lane 2 : HEK293 cell extract
Lysates/proteins at 2 µg/ml per lane.
Predicted band size: 47 kDa
Observed band size: ~50-53 kDa why is the actual band size different from the predicted?
ab36607 at 1/1000 dilution staining FOXP3 in human PANC1 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary antibody show green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 Phalloidin.
Overlay histogram showing Jurkat cells stained with ab36607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Ji H et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 64:573-82 (2014). Read more (PubMed: 24935938) »
- Imam S et al. Diabetes-associated dry eye syndrome in a new humanized transgenic model of type 1 diabetes. Mol Vis 19:1259-67 (2013). ICC/IF ; Mouse . Read more (PubMed: 23805032) »