Product nameAnti-FOXP3 antibody
See all FOXP3 primary antibodies
DescriptionRabbit polyclonal to FOXP3
SpecificityThis antibody recognises a single clean band representing FOXP3 on HEK293 cell lysate overexpressing human FOXP3. This band is blocked by the immunising peptide and is not present in the control HEK293 cell lysate.
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ChIPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Hek 293 lysate over expressing human FOXP3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab10901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human FOXP3 peptide (ab16809).|
|IHC-P||Use a concentration of 0.3 - 1 µg/ml.|
|ICC/IF||Use a concentration of 2 - 4 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
FunctionProbable transcription factor. Plays a critical role in the control of immune response.
Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
- Information by UniProt
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
Western blot using ab10901 at 1/500.
Lane 1: HEK 293 lysate.A no primary control lane showed no bands.
Lane 2: HEK 293 lysate over expressing human FOXP3.
Lane 3: HEK 293 lysate over expressing mouse FOXP3.
Lane 4: HEK 293 lysate with FOXP3 peptide ab16809.
Lane 5: HEK 293 lysate over expressing human FOXP3 with FOXP3 peptide ab16809.
Lane 6: HEK 293 lysate over expressing mouse FOXP3 with FOXP3 peptide ab16809.
All lanes with ab10901.
A no primary control lane showed no bands.
Peptide at 1
Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 1 minute.
Expected molecular weight: 47kD
Lysates at 20
This product has been referenced in:
- Skretting G et al. Transcription factor FOXP3: A repressor of the TFPI gene? J Cell Biochem N/A:N/A (2019). Read more (PubMed: 30861202) »
- Ma F et al. Interleukin-6-mediated CCR9+ interleukin-17-producing regulatory T cells polarization increases the severity of necrotizing enterocolitis. EBioMedicine 44:71-85 (2019). Read more (PubMed: 31129099) »