Recombinant

Recombinant Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR034F] to FOXP3 - BSA free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXP3 antibody [BLR034F] - BSA free
    See all FOXP3 primary antibodies

  • Description

    Rabbit monoclonal [BLR034F] to FOXP3 - BSA free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FOXP3 aa 275-325. The exact sequence is proprietary. NP_054728.2 and Gene ID 50943.
    Database link: Q9BZS1

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC 1/100 - 1/500.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Use 6µl/mg lysate.

Target

  • Function

    Probable transcription factor. Plays a critical role in the control of immune response.

  • Involvement in disease

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Images

    • Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      FOXP3 was immunoprecipitated from 1.0 mg MJ whole cell lysate with ab243898 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243898 at 1/1000 dilution.

      Lane 1: ab243864 IP in HeLa cell lysate.

      Lane 2: Contol IgG in MJ whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 75 seconds.

    • Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

      Western blot analysis using ab243890 at 1/1000 dilution.

      Lane 1: MJ whole cell lysate (50 µg).

      Lane 2: Jurkat whole cell lysate (50 µg).

      Lane 3: HEK-293T whole cell lysate (50 µg).

      Lane 4: MOLT-4 whole cell lysate (50 µg).

      Lane 5: RPMI 8226 whole cell lysate (50 µg).

      Lane 6: HH whole cell lysate (50 µg).

      Lane 7: C5/MJ whole cell lysate (50 µg).

      Lane 8: KG-1 whole cell lysate (50 µg).

      A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 minutes.

    • Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
    • Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
      Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243890? Please let us know so that we can cite the reference in this datasheet.

    ab243890 has not yet been referenced specifically in any publications.

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