Recombinant Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-466800-antifoxp3-antibody-BLR034F-immunohistochemistry-breast-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR034F] to FOXP3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-FOXP3 antibody [BLR034F] - BSA free
See all FOXP3 primary antibodies -
Description
Rabbit monoclonal [BLR034F] to FOXP3 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FOXP3 aa 275-325. The exact sequence is proprietary. NP_054728.2 and Gene ID 50943.
Database link: Q9BZS1 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR034F -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
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| ICC |
1/100 - 1/500.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| IP |
Use at an assay dependent concentration.
Use 6µl/mg lysate. |
| Notes |
|---|
|
WB
1/1000. |
|
ICC
1/100 - 1/500. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. Use 6µl/mg lysate. |
Target
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Function
Probable transcription factor. Plays a critical role in the control of immune response. -
Involvement in disease
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXP3 using ab243890 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-334350-anti-foxp3-antibody-blr034f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining. -
![Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-466797-ab243890-AntiFOXP3-antibody-western-blot.jpg)
Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)All lanes : Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : MJ whole cell lysate
Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : RPMI-8226 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 30 secondsDetection: Chemiluminesence
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![Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-466769-ab243890-AntiFOXP3-antibody-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)All lanes : Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1 : MJ whole cell lysate at 20 %
Lane 2 : Control IgG in MJ whole cell lysate
Exposure time: 30 seconds -
![Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-466792-antifoxp3-antibody-BLR034F-immunocytochemistry-mj-cells.jpg)
Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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![Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)](https://www.abcam.com/ps/products/243/ab243890/Images/ab243890-6-benefits-of-recombinant-antibodies.png)
Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243890 has not yet been referenced specifically in any publications.