Name: Anti-FOXP3 antibody [EPR22102-37]
Brand: RabMAb
SKU: ab215206
Rating: 5 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22102-37] to FOXP3
Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), mIHC
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-FOXP3 antibody [EPR22102-37]
See all FOXP3 primary antibodies
Description

Rabbit monoclonal [EPR22102-37] to FOXP3
Host species

Rabbit
Specificity

ab215206 failed to detect endogenous expression of FOXP3 in the rat tissues tested in WB.

According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.
Tested applications

Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), mIHCmore details
Unsuitable for: ICC/IF or IHC-Fr
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with FOXP3 (WT) expression vector with a GFP-myc tag, whole cell lysate. Full-length C-MYC/DDK tagged Recombinant Mouse Foxp3 protein, human tonsil, mammary gland, breast cancer and mouse mammary gland lysates. IHC-P: Human, mouse, rat thymus tissues, human and mouse spleen tissues, human tonsil tissue. Flow Cyt (intra): HEK-293T transfected with a GFP-tagged FOXP3 expression construct. IP: HEK-293T transfected with a GFP-tagged FOXP3 expression construct whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22102-37
Isotype

IgG
Research areas

Alternative Versions
- Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human FOXP3 protein (His tag) (ab226445)
Related Products
- Universal HIER antigen retrieval reagent (10X) (ab208572)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab215206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.
IP		1/30.
IHC-P	(5)	1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Please adjust the dilution factor according to tissues from different species. Recommend performing IHC staining on a Leica Biosystems BOND^® RX instrument because BOND^® RX instrument assay is stronger than overnight incubation assay.
Flow Cyt (Intra)		1/40.
mIHC		1/100.

Notes
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.
IP 1/30.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Please adjust the dilution factor according to tissues from different species. Recommend performing IHC staining on a Leica Biosystems BOND^® RX instrument because BOND^® RX instrument assay is stronger than overnight incubation assay.
Flow Cyt (Intra) 1/40.
mIHC 1/100.

Application notes

Is unsuitable for ICC/IF or IHC-Fr.

Function

Probable transcription factor. Plays a critical role in the control of immune response.
Involvement in disease

Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similarities

Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 50943 Human
- Entrez Gene: 20371 Mouse
- Entrez Gene: 317382 Rat
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- SwissProt: Q99JB6 Mouse
- Unigene: 247700 Human
- Unigene: 182291 Mouse
Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
- Forkhead box protein P3 antibody
- FOXP3 antibody
- FOXP3_HUMAN antibody
- FOXP3delta7 antibody
- Immune dysregulation polyendocrinopathy enteropathy X linked antibody
- Immunodeficiency polyendocrinopathy enteropathy X linked antibody
- IPEX antibody
- JM2 antibody
- MGC141961 antibody
- MGC141963 antibody
- OTTHUMP00000025832 antibody
- OTTHUMP00000025833 antibody
- OTTHUMP00000226737 antibody
- PIDX antibody
- Scurfin antibody
- XPID antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling FOXP3 with ab215206 at 1:100 dilution (3.67 μg/ml).

Image A: LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with ab215206 for 30min at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

Image B: Goat Anti-Rabbit IgG H&L (HRP polymer) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with ab215206 overnight at +4 ℃. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)This image is courtesy of TissueGnostics Asia Pacific Limited

10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Red; TG700N), anti-CD163 (ab182422; Brown; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Violet; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain. The inset image shows the separate FOXP3 signal.

The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).
Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)This image is courtesy of TissueGnostics Asia Pacific Limited

10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Violet; TG700N), anti-CD163 (ab182422; Red; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Orange; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain.

The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).
Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

All lanes : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/1000 dilution

Lane 1 : Human tonsil tissue lysate
Lane 2 : Human mammary gland tissue lysate
Lane 3 : Human breast cancer tissue lysate
Lane 4 : Mouse mammary gland tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time : Lanes 1 & 2: 26 seconds. Lanes 3 & 4: 3 minutes.

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with a GFP-tagged FOXP3 expression construct labeling FOXP3 with ab215206 at 1/40 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) (ab150079), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Lane 1 : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/200 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution (Anti-DDDDK tag, 1:1000 dilution)

All lanes : Full-length C-MYC/DDK tagged Recombinant Mouse Foxp3 protein 10ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling FOXP3 with ab215206 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

FOXP3 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate with ab215206 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215206 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate 10 μg (Input).
Lane 2: ab215206 IP in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215206 in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.

FOXP3 isoforms have been described in the literature. (PMID: 19568423).
Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

All lanes : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-myc tag, whole cell lysate
Lane 2 : HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 47 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?

Exposure time: 6 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Human PBMCs were stained with Alexa Fluor^® 647 conjugated anti-human CD4 and BV421 conjugated anti-human CD25. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab98462, Dylight^®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.

Gating strategy and expression pattern is consistent with literature (PMID: 27330808).
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Mouse splenocytes were stained with Alexa Fluor^® 647 conjugated anti-mouse CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilisation buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.
Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Rat splenocytes were stained with Alexa Fluor^® 647 conjugated anti-rat CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab150077, Alexa Fluor^®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in rat thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in mouse spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in mouse thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FOXP3 with ab215206 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Anti-FOXP3 antibody [EPR22102-37] (ab215206)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab215206? Please let us know so that we can cite the reference in this datasheet.

ab215206 has been referenced in 15 publications.

Wang F et al. Discovery of a new inhibitor targeting PD-L1 for cancer immunotherapy. Neoplasia 23:281-293 (2021). PubMed: 33529880
He HX et al. VISTA and PD-L1 synergistically predict poor prognosis in patients with extranodal natural killer/T-cell lymphoma. Oncoimmunology 10:1907059 (2021). PubMed: 33889438
Wang Y et al. Tanshinone IIA regulates microRNA-125b/foxp3/caspase-1 signaling and inhibits cell viability of nasopharyngeal carcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33760137
Duan XC et al. The synergistic antitumor activity of 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs) and anti-PD-L1 antibody inducing immunogenic cell death. Drug Deliv 28:800-813 (2021). PubMed: 33866918
Chen KT et al. Neuronavigation-guided focused ultrasound for transcranial blood-brain barrier opening and immunostimulation in brain tumors. Sci Adv 7:N/A (2021). PubMed: 33547073

View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (palatine tonsil)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Specification

palatine tonsil

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 05 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Specification

Spleen

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

Submitted Dec 31 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Lymph node)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Yes - H2O2

Specification

Lymph node

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 19 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Leica ER2 solution

Specification

Tumor

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Zohar Gavish

Verified customer

Submitted May 13 2019

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Key features and details

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Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (15)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]