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    foxp3-antibody-epr22102-37-ab215206.pdf

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RecombinantRabMAb

Recombinant Anti-FOXP3 antibody [EPR22102-37] (ab215206)

  • Datasheet
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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
  • Anti-FOXP3 antibody [EPR22102-37] (ab215206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22102-37] to FOXP3
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), mIHC
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-FOXP3 antibody [EPR22102-37]
    See all FOXP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22102-37] to FOXP3
  • Host species

    Rabbit
  • Specificity

    ab215206 failed to detect endogenous expression of FOXP3 in the rat tissues tested in WB.

    According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus. It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), mIHCmore details
    Unsuitable for: ICC/IF or IHC-Fr
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with FOXP3 (WT) expression vector with a GFP-myc tag, whole cell lysate. Full-length C-MYC/DDK tagged Recombinant Mouse Foxp3 protein, human tonsil, mammary gland, breast cancer and mouse mammary gland lysates. IHC-P: Human, mouse, rat thymus tissues, human and mouse spleen tissues, human tonsil tissue. Flow Cyt (intra): HEK-293T transfected with a GFP-tagged FOXP3 expression construct. IP: HEK-293T transfected with a GFP-tagged FOXP3 expression construct whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22102-37
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (ab244242)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human FOXP3 protein (His tag) (ab226445)
  • Related Products

    • Universal HIER antigen retrieval reagent (10X) (ab208572)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab215206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus.  It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.

IP
1/30.
IHC-P (5)
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please adjust the dilution factor according to tissues from different species.

Recommend performing IHC staining on a Leica Biosystems BOND® RX instrument because BOND® RX instrument assay is stronger than overnight incubation assay.

Flow Cyt (Intra)
1/40.
mIHC
1/100.
Notes
WB
1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

According to our preliminary WB data, this antibody failed to detect endogenous expression of FOXP3 in the tissues tested in WB, such as thymus.  It might work in CD4+CD25+ Treg cells with abundant FOXP3, but we don’t have experimental data to support that.

IP
1/30.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please adjust the dilution factor according to tissues from different species.

Recommend performing IHC staining on a Leica Biosystems BOND® RX instrument because BOND® RX instrument assay is stronger than overnight incubation assay.

Flow Cyt (Intra)
1/40.
mIHC
1/100.
Application notes
Is unsuitable for ICC/IF or IHC-Fr.

Target

  • Function

    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50943 Human
    • Entrez Gene: 20371 Mouse
    • Entrez Gene: 317382 Rat
    • Omim: 300292 Human
    • SwissProt: Q9BZS1 Human
    • SwissProt: Q99JB6 Mouse
    • Unigene: 247700 Human
    • Unigene: 182291 Mouse
    • Alternative names

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling FOXP3 with ab215206 at 1:100 dilution (3.67 μg/ml).

      Image A: LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with ab215206 for 30min at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

      Image B: Goat Anti-Rabbit IgG H&L (HRP polymer) secondary antibody used at a ready to use concentration. Nuclear staining on human tonsil. The section was incubated with ab215206 overnight at +4 ℃. Counterstained with Hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)This image is courtesy of TissueGnostics Asia Pacific Limited

      10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

      Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Red; TG700N), anti-CD163 (ab182422; Brown; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Violet; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain. The inset image shows the separate FOXP3 signal.

      The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

      Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

      Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

    • Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Multiplex immunohistochemistry - Anti-FOXP3 antibody [EPR22102-37] (ab215206)This image is courtesy of TissueGnostics Asia Pacific Limited

      10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

      Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Violet; TG700N), anti-CD163 (ab182422; Red; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Orange; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain.

      The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

      Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

      Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

    • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      All lanes : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/1000 dilution

      Lane 1 : Human tonsil tissue lysate
      Lane 2 : Human mammary gland tissue lysate
      Lane 3 : Human breast cancer tissue lysate
      Lane 4 : Mouse mammary gland tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 47 kDa



      Exposure time : Lanes 1 & 2: 26 seconds. Lanes 3 & 4: 3 minutes.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with a GFP-tagged FOXP3 expression construct labeling FOXP3 with ab215206 at 1/40 dilution (Right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079), at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Lane 1 : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/200 dilution
      Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution (Anti-DDDDK tag, 1:1000 dilution)

      All lanes : Full-length C-MYC/DDK tagged Recombinant Mouse Foxp3 protein 10ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 47 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking buffer and concentration: 5% NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling FOXP3 with ab215206 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunoprecipitation - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      FOXP3 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate with ab215206 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215206 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate 10 μg (Input).
      Lane 2: ab215206 IP in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (+). 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215206 in HEK-293T cells transfected with a GFP-tagged FOXP3 expression construct whole cell lysate (-).

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 seconds.

      FOXP3 isoforms have been described in the literature. (PMID: 19568423).

    • Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Western blot - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      All lanes : Anti-FOXP3 antibody [EPR22102-37] (ab215206) at 1/1000 dilution

      Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-myc tag, whole cell lysate
      Lane 2 : HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 47 kDa
      Observed band size: 73 kDa why is the actual band size different from the predicted?


      Exposure time: 6 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Human PBMCs were stained with Alexa Fluor® 647 conjugated anti-human CD4 and BV421 conjugated anti-human CD25. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab98462, Dylight®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000. 

      Gating strategy and expression pattern is consistent with literature (PMID: 27330808). 

       

       

    • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Mouse splenocytes were stained with Alexa Fluor® 647 conjugated anti-mouse CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilisation buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000. 

       

       

    • Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Rat splenocytes were stained with Alexa Fluor® 647 conjugated anti-rat CD4. Cells were then fixed with 2% PFA for 10min and permeabilized with True-Nuclear™ permeabilization buffer, followed by intracellular staining with rabbit IgG (ab172730, Left) or anti-FOXP3 RabMab (Right, 1/40). ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as the secondary at a dilution of 1/2000. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in rat thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in mouse spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with ab215206 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in mouse thymus (PMID: 16380964) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR22102-37] (ab215206)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FOXP3 with ab215206 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on regulatory T-cells in human spleen (PMID: 16380964) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

    • Anti-FOXP3 antibody [EPR22102-37] (ab215206)
      Anti-FOXP3 antibody [EPR22102-37] (ab215206)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (15)

    Publishing research using ab215206? Please let us know so that we can cite the reference in this datasheet.

    ab215206 has been referenced in 15 publications.

    • Wang F  et al. Discovery of a new inhibitor targeting PD-L1 for cancer immunotherapy. Neoplasia 23:281-293 (2021). PubMed: 33529880
    • He HX  et al. VISTA and PD-L1 synergistically predict poor prognosis in patients with extranodal natural killer/T-cell lymphoma. Oncoimmunology 10:1907059 (2021). PubMed: 33889438
    • Wang Y  et al. Tanshinone IIA regulates microRNA-125b/foxp3/caspase-1 signaling and inhibits cell viability of nasopharyngeal carcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33760137
    • Duan XC  et al. The synergistic antitumor activity of 3-(2-nitrophenyl) propionic acid-paclitaxel nanoparticles (NPPA-PTX NPs) and anti-PD-L1 antibody inducing immunogenic cell death. Drug Deliv 28:800-813 (2021). PubMed: 33866918
    • Chen KT  et al. Neuronavigation-guided focused ultrasound for transcranial blood-brain barrier opening and immunostimulation in brain tumors. Sci Adv 7:N/A (2021). PubMed: 33547073
    View all Publications for this product

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 13 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Hannes Kaddatz

    Verified customer

    Submitted Dec 31 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - H2O2
    Specification
    Lymph node
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [EPR22102-37]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica ER2 solution
    Specification
    Tumor
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Zohar Gavish

    Verified customer

    Submitted May 13 2019

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