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  1. Link

    foxp3-antibody-mabcam-22510-bsa-and-azide-free-ab188638.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
  • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
  • Western blot - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

Key features and details

  • Mouse monoclonal [mAbcam 22510] to FOXP3 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free
    See all FOXP3 primary antibodies
  • Description

    Mouse monoclonal [mAbcam 22510] to FOXP3 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Detects a single clean band on human FOXP3 transfected 293 cells. Gives a very similar staining pattern to the ab20034 on human tonsil, confirming its specificity for FOXP3. Detects FOXP3 in mouse samples in western blot. However, not suitable for mouse in IHC.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and spleen tissue. Human colon carcinoma tissue. IHC-Fr: Human tonsil tissue. WB: HEK293 cells overexpressing human FOXP3.
  • General notes

    ab188638 is the carrier-free version of ab22510.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 22510
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-FOXP3 antibody [mAbcam 22510] (ab22510)
    • HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human FOXP3 protein (His tag) (ab226445)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab188638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Function

    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease

    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50943 Human
    • Entrez Gene: 317382 Rat
    • Omim: 300292 Human
    • SwissProt: Q9BZS1 Human
    • Unigene: 247700 Human
    • Alternative names

      • AIID antibody
      • DIETER antibody
      • Forkhead box P3 antibody
      • Forkhead box protein P3 antibody
      • FOXP3 antibody
      • FOXP3_HUMAN antibody
      • FOXP3delta7 antibody
      • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
      • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
      • IPEX antibody
      • JM2 antibody
      • MGC141961 antibody
      • MGC141963 antibody
      • OTTHUMP00000025832 antibody
      • OTTHUMP00000025833 antibody
      • OTTHUMP00000226737 antibody
      • PIDX antibody
      • Scurfin antibody
      • XPID antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

      Image produced using the same antibody clone but in a different formulation ab22510.

      IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

      Image produced using the same antibody clone but in a different formulation ab22510.

      IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

      Image produced using the same antibody clone but in a different formulation ab22510.

      IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human spleen* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab22510, 10ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      Western blot - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      All lanes : Anti-FOXP3 antibody [mAbcam 22510] (ab22510) at 1 µg/ml

      Lane 1 : HEK293 cells overexpressing human FOXP3
      Lane 2 : HEK293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 45 seconds
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (ab188638)

      Image produced using the same antibody clone but in a different formulation ab22510.

      IHC image of ab22510 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22510, 20µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab188638? Please let us know so that we can cite the reference in this datasheet.

    ab188638 has not yet been referenced specifically in any publications.

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