Product nameAnti-FOXP3 antibody [mAbcam 22510] (HRP)
See all FOXP3 primary antibodies
DescriptionMouse monoclonal [mAbcam 22510] to FOXP3 (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
Clone numbermAbcam 22510
Light chain typekappa
Immunizing Peptide (Blocking)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab22831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human FOXP3 peptide (ab16809).|
FunctionProbable transcription factor. Plays a critical role in the control of immune response.
Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
- Information by UniProt
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
All lanes : Anti-FOXP3 antibody [mAbcam 22510] (HRP) (ab22831) at 1/1000 dilution
Lane 1 : HEK 293 cells overexpressing human FOXP3
Lane 2 : HEK 293 cells overexpressing human FOXP3 with
Human FOXP3 peptide (ab16809) at 1 µg/ml
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes
ab22831 staining FOXP3 in human tonsil tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocked (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C.
ab22831 has not yet been referenced specifically in any publications.