Anti-FOXP3 antibody [mAbcam 450] (ab450)
- Datasheet
- References (17)
- Protocols
Overview
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Product nameAnti-FOXP3 antibody [mAbcam 450]
See all FOXP3 primary antibodies -
DescriptionMouse monoclonal [mAbcam 450] to FOXP3
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Host speciesMouse
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SpecificityDetects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.
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Tested applicationsSuitable for: IHC-Fr, IP, ICC, IHC-P, ICC/IF, WBmore details
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Species reactivityReacts with: Human
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Immunogen
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Positive control
- IHC-P: FFPE Human tonsil tissue sections. IHC-Fr: Human tonsil tissue sections.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numbermAbcam 450
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MyelomaSp2/0-Ag14
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IsotypeIgG3
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 5 µg/ml. | |
| IP | Use at an assay dependent concentration. Used at 2 µg/ml on human FOXP3 transfected COS cells (see Abreview for further information). | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | 1/50. This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer. It did not show positive staining with cryostat or paraffin-embedded human tonsil. |
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| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXP3 peptide (ab16809). |
Target
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FunctionProbable transcription factor. Plays a critical role in the control of immune response.
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Involvement in diseaseDefects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
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Sequence similaritiesContains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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Alternative names
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
Images
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![Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)](https://www.abcam.com/ps/products/0/ab450/Images/ab450_1.jpg)
Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)All lanes : Anti-FOXP3 antibody [mAbcam 450] (ab450) at 1 µg/ml
Lane 1 : HEK293 cells overexpressing human FOXP3
Lane 2 : HEK293 cells overexpressing human FOXP3 withHuman FOXP3 peptide (ab16809) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDa
Exposure time: 45 seconds -
![Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)](https://www.abcam.com/ps/products/0/ab450/Images/ab450_5.jpg)
Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)This image is courtesy of Alison Banham and Bridget Fox, University of OxfordClone mAbcam 450 used in immunocytochemistry on paraffin embedded transfected COS-1 cytospins.
1: FOXP1 transfectant
2: FOXP2 transfectant
3: FOXP3 transfectant
4: FOXP4 transfectant
Strong staining can be seen on the FOXP3 transfectant, with very weak staining on the FOXP1 transfectant and no staining is seen with the other FOXP protein transfectants. This shows that the antibody is largely specific for FOXP3 when used with paraffin embedded cells.
These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant). -
![Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)](https://www.abcam.com/ps/products/0/ab450/Images/ab450-312938-ab450AP3665863hutonsilfrozen10ugER1.jpg)
Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab450, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)](https://www.abcam.com/ps/products/0/ab450/Images/ab450_2.jpg)
Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)This image is courtesy of Alison Banham and Bridget Fox, University of OxfordClone mAbcam 450 used in immunocytochemistry on transfected COS-1 cytospins fixed with acetone for 10 mins.
1: FOXP1 transfectant
2: FOXP2 transfectant
3: FOXP3 transfectant
4: FOXP4 transfectant
Staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants, confirming the specificity of this antibody in immunocytochemistry.
These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)](https://www.abcam.com/ps/products/0/ab450/Images/ab450-312937-ab450AP3665863hutonsil10ugER1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab450, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Protocols
Datasheets and documents
References
This product has been referenced in:
- Mu L et al. The IDH1 Mutation-Induced Oncometabolite, 2-Hydroxyglutarate, May Affect DNA Methylation and Expression of PD-L1 in Gliomas. Front Mol Neurosci 11:82 (2018). Read more (PubMed: 29643764) »
- Jiang H et al. Abnormal acetylation of FOXP3 regulated by SIRT-1 induces Treg functional deficiency in patients with abdominal aortic aneurysms. Atherosclerosis 271:182-192 (2018). IP . Read more (PubMed: 29524861) »
