Recombinant

Anti-FOXP3 antibody [SP97] (ab99963)

Overview

  • Product name
    Anti-FOXP3 antibody [SP97]
    See all FOXP3 primary antibodies
  • Description
    Rabbit monoclonal [SP97] to FOXP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human FOXP3 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9BZS1

  • Positive control
    • IHC-P: Human tonsil, pancreatic adenocarcinoma, stomach adenocarcinoma, lymph node, B cell lymphoma, colon, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, thymus and cervical squamous cell carcinoma.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab99963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Boil tissue section in 10mM Citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    Probable transcription factor. Plays a critical role in the control of immune response.
  • Involvement in disease
    Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIID antibody
    • DIETER antibody
    • Forkhead box P3 antibody
    • Forkhead box protein P3 antibody
    • FOXP3 antibody
    • FOXP3_HUMAN antibody
    • FOXP3delta7 antibody
    • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
    • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
    • IPEX antibody
    • JM2 antibody
    • MGC141961 antibody
    • MGC141963 antibody
    • OTTHUMP00000025832 antibody
    • OTTHUMP00000025833 antibody
    • OTTHUMP00000226737 antibody
    • PIDX antibody
    • Scurfin antibody
    • XPID antibody
    see all

Images

  • Immunohistochemical analysis of Human Tonsil tissue labelling FOXP3 with ab99963.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lymph node tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B cell lymphoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Leduc C  et al. TPF induction chemotherapy increases PD-L1 expression in tumour cells and immune cells in head and neck squamous cell carcinoma. ESMO Open 3:e000257 (2018). Read more (PubMed: 29344407) »
  • Tiwari V  et al. CD4+ CD25+ FOXP3+ T cell frequency in the peripheral blood is a biomarker that distinguishes intestinal tuberculosis from Crohn's disease. PLoS One 13:e0193433 (2018). IHC-P ; Human . Read more (PubMed: 29489879) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 Solution
Permeabilization
No
Specification
Mouse Spleen
Blocking step
(agent) for 12 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
10% NBF
Username

Jenna Vandervolgen

Verified customer

Submitted Aug 13 2018

For IHC-P staining with product ab99963, Human Tonsil Samples were formalin fixed.

Our full IHC-P fixation protocol can be found here: IHC Fixation Protocol.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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