• Product name

  • Description

    Rabbit polyclonal to FOXQ1
  • Host species

  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: GDDSLGSDGD CAANSPAAGG GARDPPGDGE QSAGGGPGAE EAIPAAAAAA, corresponding to N terminal amino acids 36-85 of Human FOXQ1

  • Positive control

    • Jurkat cell lysate and human kidney tissue.



Our Abpromise guarantee covers the use of ab51340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • Tissue specificity

    Expressed predominantly in the stomach, trachea, bladder and salivary gland.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Forkhead box protein Q1 antibody
    • Forkhead box Q1 antibody
    • FOX Q1 antibody
    • FOXQ 1 antibody
    • FOXQ1 antibody
    • FOXQ1_HUMAN antibody
    • Hepatocyte nuclear factor 3 forkhead antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 1 antibody
    • HFH 1 antibody
    • HFH-1 antibody
    • HFH1 antibody
    • HNF 3/forkhead like protein 1 antibody
    • HNF-3/forkhead-like protein 1 antibody
    • HNF3/forkhead like protein 1 antibody
    • MGC77469 antibody
    • Q1 antibody
    • Winged helix / forkhead transcription factor antibody
    • Winged helix/forkhead transcription factor antibody
    • zgc:77469 antibody
    see all


  • Anti-FOXQ1 antibody (ab51340) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS at 5 %

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 41 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXQ1 with ab51340 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells of renal tubule. Magnification: 400X.

  • ICC/IF image of ab51340 stained cells. The cells were and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Qin A  et al. MicroRNA-1271 inhibits cellular proliferation of hepatocellular carcinoma. Oncol Lett 14:6783-6788 (2017). Read more (PubMed: 29181102) »
  • Yonemori K  et al. The microRNA expression signature of pancreatic ductal adenocarcinoma by RNA sequencing: anti-tumour functions of themicroRNA-216cluster. Oncotarget 8:70097-70115 (2017). Read more (PubMed: 29050264) »
See all 6 Publications for this product

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