Key features and details
- Rabbit polyclonal to FOXR2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab55961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Tissue specificityExpressed in breast cancer cell lines and primary cancer.
Sequence similaritiesContains 1 fork-head DNA-binding domain.
- Information by UniProt
- Forkhead box protein N6 antibody
- Forkhead box protein R2 antibody
- FOXN6 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling FOXR2 with ab55961 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.
Lane 1 : marker
Lane 2 : Anti-FOXR2 antibody (ab55961) at 1.25 µg/ml
Lane 2 : Jurkat cell lysate at 10 µg
Lane 2 : HRP-labelled anti-rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab55961 has not yet been referenced specifically in any publications.