Overview

  • Product name
  • Description
    Rabbit polyclonal to FPGS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FPGS aa 310-339 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q05932

  • Positive control
    • K562 and A2058 cell line lysates; Human lung carcinoma tissue; K562 cells.
  • General notes

    Previously labelled as Folylpolyglutamate synthase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab175849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Conversion of folates to polyglutamate derivatives. This allows tissues to concentrate folate at higher levels than in plasma.
  • Pathway
    Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
  • Sequence similarities
    Belongs to the folylpolyglutamate synthase family.
  • Cellular localization
    Mitochondrion. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 6.3.2.17 antibody
    • FOLC antibody
    • FOLC_HUMAN antibody
    • Folylpoly gamma glutamate synthetase antibody
    • Folylpoly-gamma-glutamate synthetase antibody
    • Folylpolyglutamate synthase antibody
    • Folylpolyglutamate synthase mitochondrial antibody
    • FPGS antibody
    • mitochondrial antibody
    • OTTHUMP00000022201 antibody
    • OTTHUMP00000022202 antibody
    • Tetrahydrofolate synthase antibody
    • Tetrahydrofolylpolyglutamate synthase antibody
    see all

Images

  • All lanes : Anti-FPGS antibody (ab175849) at 1/1000 dilution

    Lane 1 : K562 cell line lysate
    Lane 2 : A2058 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 65 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling FPGS with ab175849 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells labeling FPGS with ab175849 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175849 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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