Key features and details
- Rabbit polyclonal to FPGS
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-FPGS antibody
See all FPGS primary antibodies
DescriptionRabbit polyclonal to FPGS
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment (His-T7-tag) corresponding to Mouse FPGS aa 210-439. (Expressed in E.coli).
TNIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQP EGPLAVLRDRAQQIGCPLYLCPPLEALEEVGLPLSLGLEGAHQRSNAALA LQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAPVFRPTPHMRRGLRDTV WPGRTQILQRGPLTWYLDGAHTTSSVQACVHWYRQSLERSKRTDGGSEVH ILLFNSTGDRDSAALLKLLQPCQFDYAVFC
Database link: P48760
- WB: Recombinant mouse FPGS and HeLa whole cell lysate. IHC-P: Mouse kidney and skeletal muscle tissue.
Previously labelled as Folylpolyglutamate synthase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 65 kDa.|
FunctionConversion of folates to polyglutamate derivatives. This allows tissues to concentrate folate at higher levels than in plasma.
PathwayCofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.
Sequence similaritiesBelongs to the folylpolyglutamate synthase family.
Cellular localizationMitochondrion. Cytoplasm.
- Information by UniProt
- EC 18.104.22.168 antibody
- FOLC antibody
- FOLC_HUMAN antibody
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for FPGS using ab231540 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse skeletal muscle tissue stained for FPGS using ab231540 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Anti-FPGS antibody (ab231540) at 1 µg/ml + Recombinant mouse FPGS protein
Predicted band size: 65 kDa
Anti-FPGS antibody (ab231540) at 1/50 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 65 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231540 has not yet been referenced specifically in any publications.