Overview

  • Product name
    Anti-FPRL1 antibody [GM1D6]
    See all FPRL1 primary antibodies
  • Description
    Mouse monoclonal [GM1D6] to FPRL1
  • Host species
    Mouse
  • Specificity
    Ab26316 recognises FPRL1 transiently expressed on the cell surface of transfected BOSC cells.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Horse, Human
  • Immunogen

    Ab26316 was generated by genetic immunisation of BALB/c mice with a proprietary GPCR-immunisation vector containing the FPRL1 cDNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab26316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 1µg for 106 cells.
IHC-Fr Use at an assay dependent concentration. PubMed: 22384219
Flow Cyt Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • Tissue specificity
    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALXR antibody
    • FMLP-R-I antibody
    • FMLP-R-II antibody
    • FMLP-related receptor I antibody
    • FMLPX antibody
    • Formyl peptide receptor 2 antibody
    • Formyl peptide receptor related antibody
    • Formyl peptide receptor-like 1 antibody
    • FPR2 antibody
    • FPR2_HUMAN antibody
    • FPR2A antibody
    • FPRH1 antibody
    • FPRH2 antibody
    • FPRL1 antibody
    • HM63 antibody
    • Lipoxin A4 receptor antibody
    • LXA4 receptor antibody
    • LXA4R antibody
    • N-formyl peptide receptor 2 antibody
    • RFP antibody
    see all

Images

  • FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1 (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.
  • ab26316 staining of FPRL1 in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1 and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

  • ab26316, staining FPRL1 (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).

    After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).

References

This product has been referenced in:
  • Herteman N  et al. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses. Sci Rep 7:7743 (2017). IF ; Horse . Read more (PubMed: 28798364) »
  • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Tendon Derived Cells)
Permeabilization
No
Specification
Tendon Derived Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde

Ms. Giulia Sivelli

Verified customer

Submitted Sep 17 2018

Answer

Thank you for your enquiry and your interest in our products.

The antibody ab26316 recognizes the extracellular part of the receptor. We tested this in FACS on transiently transfected cells. Some customers tested the antibody and its ability to block the receptor:

Here are some useful and specific publications you may wish to take a look at:

Resolvin D1 Limits Polymorphonuclear Leukocyte Recruitment to Inflammatory Loci : Receptor-Dependent Actions. Lucy V. Norling et al, Arteriosclerosis, Thrombosis, and Vascular Biology. 2012; 32: 1970-1978

Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Carmela De Santo et al, Nature Immunology VOLUME 11 NUMBER 11 NOVEMBER 2010

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for contacting us.

Antibodies are sometimes pre-adsorbed. This means they have been adsorbed with other proteins, or serum from various species, etc, to eliminate any antibody that may cross-react. The resulting purified antibody should be very pure and specific and any cross reactivity should be significantly reduced.

As shown on the datasheet, the primary antibody ab26316 is a Mouse IgG1. Therefore I confirm that ab99605 is compatible with ab26316.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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