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Ab26316 was generated by genetic immunisation of BALB/c mice with a proprietary GPCR-immunisation vector containing the FPRL1 cDNA.
Our Abpromise guarantee covers the use of ab26316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use 1µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22384219|
|Flow Cyt||Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab26316 staining of FPRL1 in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1 and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"