Anti-FPRL1/RFP antibody (ab203129)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FPRL1/RFP antibody
See all FPRL1/RFP primary antibodies -
Description
Rabbit polyclonal to FPRL1/RFP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FPRL1/RFP aa 84-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:SMAMGEKWPFGWFLCKL
Database link: P25090 -
Positive control
- Human pneumonitis and injured rat spinal cord tissues; Mouse splenocytes.
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General notes
This product was previously labelled as FPRL1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol
Aqueous buffered solution. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. (or 1/50 - 1/200 using a fluorescent secondary antibody). |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 39 kDa. | |
| Flow Cyt | 1/20 - 1/100. |
Target
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Function
Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). -
Tissue specificity
Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2358 Human
- Entrez Gene: 14294 Mouse
- Entrez Gene: 681706 Rat
- Omim: 136538 Human
- SwissProt: P25090 Human
- SwissProt: O08790 Mouse
- Unigene: 99855 Human
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Alternative names
- ALXR antibody
- FMLP-R-I antibody
- FMLP-R-II antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP antibody (ab203129)Immunohistochemical analysis of formalin-fixed, paraffin-embedded injured rat spinal cord tissue labeling FPRL1/RFP with ab203129 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Alexa Fluor 647 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FPRL1/RFP antibody (ab203129)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pneumonitis tissue labeling FPRL1/RFP with ab203129 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometry - Anti-FPRL1/RFP antibody (ab203129)Flow cytometric analysis of Mouse splenocytes labeling FPRL1/RFP with ab203129 at 1/50 dilution, followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 dilution (green) compared to control cells (blue).
Datasheets and documents
References
ab203129 has not yet been referenced specifically in any publications.
