Anti-FPRL1/RFP antibody (ab63022)
Key features and details
- Rabbit polyclonal to FPRL1/RFP
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FPRL1/RFP antibody
See all FPRL1/RFP primary antibodies -
Description
Rabbit polyclonal to FPRL1/RFP -
Host species
Rabbit -
Specificity
This antibody recognizes human FPRL-1. -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FPRL1/RFP conjugated to keyhole limpet haemocyanin.
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Positive control
- HL60 cells and extracts from HL60 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
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Flow Cyt |
1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF |
1/500.
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Notes |
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WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa). |
Flow Cyt
1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/500. |
Target
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Function
Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). -
Tissue specificity
Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2358 Human
- Omim: 136538 Human
- SwissProt: P25090 Human
- Unigene: 99855 Human
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Alternative names
- ALXR antibody
- FMLP-R-I antibody
- FMLP-R-II antibody
see all
Images
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Flow cytometric analysis of HL60 cells using ab63022 at 1:100 (red) compared to a non-specific negative control antibody (black).
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Anti-FPRL1/RFP antibody (ab63022) at 1/5000 dilution + Extracts from HL60 cells.
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of HL60 cells using ab63022 (green) at 1:500. Nucleus have bee labeled with PI (red).
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab63022 has been referenced in 6 publications.
- Li S et al. Suppression of FPR2 expression inhibits inflammation in preeclampsia by improving the biological functions of trophoblast via NF-κB pathway. J Assist Reprod Genet 39:239-250 (2022). PubMed: 35018584
- Lannan KL et al. Maresin 1 induces a novel pro-resolving phenotype in human platelets. J Thromb Haemost 15:802-813 (2017). PubMed: 28079976
- Yu N et al. Serum amyloid A, an acute phase protein, stimulates proliferative and proinflammatory responses of keratinocytes. Cell Prolif 50:N/A (2017). PubMed: 27910163
- Hsiao HM et al. Resolvin D1 Reduces Emphysema and Chronic Inflammation. Am J Pathol 185:3189-201 (2015). PubMed: 26468975
- Wood MP et al. The HIV-1 gp41 ectodomain is cleaved by matriptase to produce a chemotactic peptide that acts through FPR2. Immunology 142:474-83 (2014). PubMed: 24617769
- Gori I et al. Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4). Fertil Steril 99:1965-73.e2 (2013). PubMed: 23472950