Overview

  • Product name

  • Description

    Rabbit polyclonal to FPRL1/RFP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human FPRL1/RFP (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • HL60 cells and HL60 cell extract.
  • General notes

     This product was previously labelled as FPRL1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab63023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/6000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
ICC/IF 1/600.

Target

  • Function

    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • Tissue specificity

    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALXR antibody
    • FMLP-R-I antibody
    • FMLP-R-II antibody
    • FMLP-related receptor I antibody
    • FMLPX antibody
    • Formyl peptide receptor 2 antibody
    • Formyl peptide receptor related antibody
    • Formyl peptide receptor-like 1 antibody
    • FPR2 antibody
    • FPR2_HUMAN antibody
    • FPR2A antibody
    • FPRH1 antibody
    • FPRH2 antibody
    • FPRL1 antibody
    • HM63 antibody
    • Lipoxin A4 receptor antibody
    • LXA4 receptor antibody
    • LXA4R antibody
    • N-formyl peptide receptor 2 antibody
    • RFP antibody
    see all

Images

  • Anti-FPRL1/RFP antibody (ab63023) at 1/6000 dilution + Extracts of HL60 cells

    Predicted band size: 39 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of HL60 cells using ab63023 (green) at 1:600. Nucleus have been labeled with PI (red).

References

This product has been referenced in:

  • Xu C  et al. CSA13 inhibits colitis-associated intestinal fibrosis via a formyl peptide receptor like-1 mediated HMG-CoA reductase pathway. Sci Rep 7:16351 (2017). Read more (PubMed: 29180648) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (spleen)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
80 µg
Specification
spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Xiuzhe Wang

Verified customer

Submitted Jan 21 2012

Question
Answer

Thank you for your email.  I've looked into this, and unfortunately ab114357 would not work for blocking ab63023, as the immunogen of the antibody is amino acids 339-351, while the peptide you mentioned (ab114357) corresponds to amino acids 163-205 of FPRL1.  Since the immunogen does not overlap with the peptide sequence, the antibody will not bind to this peptide. I am sorry I could not be more helpful.  Please do not hesitate to contact us if you have any further questions.

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Question
Answer

Thank you for your patience while we investigated this. Unfortunately we are unable to source the blocking peptide for this antibody, so we are unable to provide a blocking peptide. I am sorry I could not be more helpful. Please do not hesitate to contact us if we can be of any further assistanc

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Answer

Thank you for your inquiry. We haven't tested ab63022 or ab63023 in mouse so we do not know if these antibodies will work in mouse. I've BLASTed the immunogen sequences against mouse and the results are below: Ab63022 was immunized against the 2nd extracelluar domain of FPRL-1. This sequence is only 50% homologous with mouse. Ab63023 was immunized against the C-terminal sequence of FPRL-1. This sequence is only 61% homologous with mouse. We typically say that anything with > 85% sequence homology would be expected to cross-react, therefore the chances are a bit slim with these antibodies. However, we do have a testing discount program which you could participate in to test ab63023 (the one with a greater homology) with mouse. To our knowledge, ab63023 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab63023 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab63023 in mouse. I will then send a discount code. This code must be issued before purchasing ab63023 so please wait for my reply before ordering. 2. Purchase ab63023 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63023 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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