Key features and details
- Mouse monoclonal [GM1D6] to FPRL1/RFP
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Horse, Human
- Isotype: IgG1
Product nameAnti-FPRL1/RFP antibody [GM1D6]
See all FPRL1/RFP primary antibodies
DescriptionMouse monoclonal [GM1D6] to FPRL1/RFP
Ab26316 recognises FPRL1/RFP transiently expressed on the cell surface of transfected BOSC cells.
Tested applicationsSuitable for: ICC/IF, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Horse, Human
Chemical/ Small Molecule corresponding to Human FPRL1/RFP.
This product was previously labelled as FPRL1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab26316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use 1µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22384219|
|Flow Cyt||Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionLow affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
Tissue specificityExpressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- ALXR antibody
- FMLP-R-I antibody
- FMLP-R-II antibody
FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1/RFP (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.
ab26316 staining of FPRL1/RFP in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1/RFP and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
ab26316, staining FPRL1/RFP (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).
After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).
ab26316 has been referenced in 6 publications.
- Dakin SG et al. 15-Epi-LXA4 and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture. FASEB J N/A:fj201900196R (2019). PubMed: 30916999
- Herteman N et al. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses. Sci Rep 7:7743 (2017). IF ; Horse . PubMed: 28798364
- Hsiao HM et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). PubMed: 25320283
- Dakin SG et al. Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair. PLoS One 7:e32333 (2012). IHC-Fr ; Horse . PubMed: 22384219
- Dakin SG et al. Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease. PLoS One 7:e48978 (2012). IHC-Fr ; Horse . PubMed: 23155437
- Björkman L et al. Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. J Leukoc Biol 83:245-53 (2008). PubMed: 17984291