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  1. Link

    fprl1rfp-antibody-gm1d6-ab26316.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

  • Datasheet
Reviews (1)Q&A (2)References (8)

Product price, shipping and contact information

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Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
  • Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

Key features and details

  • Mouse monoclonal [GM1D6] to FPRL1/RFP
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human FPRL1/RFP protein (ab114357)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
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Anti-Annexin A1/ANXA1 antibody [EPR19342] (ab214486)

View more associated products

Overview

  • Product name

    Anti-FPRL1/RFP antibody [GM1D6]
    See all FPRL1/RFP primary antibodies
  • Description

    Mouse monoclonal [GM1D6] to FPRL1/RFP
  • Host species

    Mouse
  • Specificity

    Ab26316 recognises FPRL1/RFP transiently expressed on the cell surface of transfected BOSC cells.

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: BOSC23 cells (Human) ICC: CHO cells transfected with an expession vector encoding FPRL1/RFP
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    GM1D6
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human FPRL1/RFP protein (ab114357)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab26316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use 1µg for 106 cells.
Flow Cyt
Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
ICC/IF
Use 1µg for 106 cells.
Flow Cyt
Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • Tissue specificity

    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P25090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2358 Human
    • Omim: 136538 Human
    • SwissProt: P25090 Human
    • Unigene: 99855 Human
    • Alternative names

      • ALXR antibody
      • FMLP-R-I antibody
      • FMLP-R-II antibody
      • FMLP-related receptor I antibody
      • FMLPX antibody
      • Formyl peptide receptor 2 antibody
      • Formyl peptide receptor related antibody
      • Formyl peptide receptor-like 1 antibody
      • FPR2 antibody
      • FPR2_HUMAN antibody
      • FPR2A antibody
      • FPRH1 antibody
      • FPRH2 antibody
      • FPRL1 antibody
      • HM63 antibody
      • Lipoxin A4 receptor antibody
      • LXA4 receptor antibody
      • LXA4R antibody
      • N-formyl peptide receptor 2 antibody
      • RFP antibody
      see all

    Images

    • Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
      Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

      FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1/RFP (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
      Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

      ab26316 staining of FPRL1/RFP in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1/RFP and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (8)

    Publishing research using ab26316? Please let us know so that we can cite the reference in this datasheet.

    ab26316 has been referenced in 8 publications.

    • Yu Y  et al. The G-protein-coupled chemoattractant receptor Fpr2 exacerbates neuroglial dysfunction and angiogenesis in diabetic retinopathy. FASEB Bioadv 2:613-623 (2020). PubMed: 33089077
    • Dakin SG  et al. 15-Epi-LXA4 and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture. FASEB J N/A:fj201900196R (2019). PubMed: 30916999
    • Dakin SG  et al. Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears. Am J Pathol 189:2258-2268 (2019). PubMed: 31437425
    • Herteman N  et al. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses. Sci Rep 7:7743 (2017). IF ; Horse . PubMed: 28798364
    • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). PubMed: 25320283
    • Dakin SG  et al. Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair. PLoS One 7:e32333 (2012). IHC-Fr ; Horse . PubMed: 22384219
    • Dakin SG  et al. Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease. PLoS One 7:e48978 (2012). IHC-Fr ; Horse . PubMed: 23155437
    • Björkman L  et al. Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. J Leukoc Biol 83:245-53 (2008). PubMed: 17984291

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FPRL1 antibody [GM1D6]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Tendon Derived Cells)
    Permeabilization
    No
    Specification
    Tendon Derived Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Giulia Sivelli

    Verified customer

    Submitted Sep 17 2018

    Question

    Inquiry: I was looking to purchase the above antibody (FPRL1, GM1D6, ab26316). I was wondering if you could tell me if this antibody binds to the extracellular part of the receptor? As i would like to investigate surface expression of this receptor using FACS. Also could you recommend a Human IgG block to use? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2012

    Answer

    Thank you for your enquiry and your interest in our products.

    The antibody ab26316 recognizes the extracellular part of the receptor. We tested this in FACS on transiently transfected cells. Some customers tested the antibody and its ability to block the receptor:

    Here are some useful and specific publications you may wish to take a look at:

    Resolvin D1 Limits Polymorphonuclear Leukocyte Recruitment to Inflammatory Loci : Receptor-Dependent Actions. Lucy V. Norling et al, Arteriosclerosis, Thrombosis, and Vascular Biology. 2012; 32: 1970-1978

    Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Carmela De Santo et al, Nature Immunology VOLUME 11 NUMBER 11 NOVEMBER 2010

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Nov 14 2012

    Question

    Inquiry: Hello, I was wondering if you could tell me what it means when it says the antibody is pre absorbed? Also can you tell me if this is compatible as a secondary for the the antibody ab26316.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2012

    Answer

    Thank you for contacting us.

    Antibodies are sometimes pre-adsorbed. This means they have been adsorbed with other proteins, or serum from various species, etc, to eliminate any antibody that may cross-react. The resulting purified antibody should be very pure and specific and any cross reactivity should be significantly reduced.

    As shown on the datasheet, the primary antibody ab26316 is a Mouse IgG1. Therefore I confirm that ab99605 is compatible with ab26316.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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