Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

Reviews (1)Q&A (2)References (5)

Overview

  • Product name
    Anti-FPRL1/RFP antibody [GM1D6]
    See all FPRL1/RFP primary antibodies
  • Description
    Mouse monoclonal [GM1D6] to FPRL1/RFP
  • Host species
    Mouse
  • Specificity

    Ab26316 recognises FPRL1/RFP transiently expressed on the cell surface of transfected BOSC cells.

  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Horse, Human
  • Immunogen

    Chemical/ Small Molecule corresponding to Human FPRL1/RFP.

Properties

Applications

Our Abpromise guarantee covers the use of ab26316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 1µg for 106 cells.
IHC-Fr Use at an assay dependent concentration. PubMed: 22384219
Flow Cyt Use 1.2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).
  • Tissue specificity
    Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • ALXR antibody
      • FMLP-R-I antibody
      • FMLP-R-II antibody
      • FMLP-related receptor I antibody
      • FMLPX antibody
      • Formyl peptide receptor 2 antibody
      • Formyl peptide receptor related antibody
      • Formyl peptide receptor-like 1 antibody
      • FPR2 antibody
      • FPR2_HUMAN antibody
      • FPR2A antibody
      • FPRH1 antibody
      • FPRH2 antibody
      • FPRL1 antibody
      • HM63 antibody
      • Lipoxin A4 receptor antibody
      • LXA4 receptor antibody
      • LXA4R antibody
      • N-formyl peptide receptor 2 antibody
      • RFP antibody
      see all

    Images

    • Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
      Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

      FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1/RFP (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
      Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)

      ab26316 staining of FPRL1/RFP in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1/RFP and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

    • Immunohistochemistry (Frozen sections) - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
      Immunohistochemistry (Frozen sections) - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)Image from Dakin SG et al., PLoS One. 2012;7(2):e32333. Epub 2012 Feb 22. Fig 6.; doi:10.1371/journal.pone.0032333; February 22, 2012, PLoS ONE 7(2): e32333.

      ab26316, staining FPRL1/RFP (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).

      After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).

    References

    This product has been referenced in:
    • Herteman N  et al. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses. Sci Rep 7:7743 (2017). IF ; Horse . Read more (PubMed: 28798364) »
    • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »
    See all 5 Publications for this product

    Publishing research using ab26316? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-FPRL1 antibody [GM1D6]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Tendon Derived Cells)
    Permeabilization
    No
    Specification
    Tendon Derived Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More

    Ms. Giulia Sivelli

    Verified customer

    Submitted Sep 17 2018

    Question

    Inquiry: I was looking to purchase the above antibody (FPRL1, GM1D6, ab26316). I was wondering if you could tell me if this antibody binds to the extracellular part of the receptor? As i would like to investigate surface expression of this receptor using FACS. Also could you recommend a Human IgG block to use? Thanks

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    Answer

    Thank you for your enquiry and your interest in our products.

    The antibody ab26316 recognizes the extracellular part of the receptor. We tested this in FACS on transiently transfected cells. Some customers tested the antibody and its ability to block the receptor:

    Here are some useful and specific publications you may wish to take a look at:

    Resolvin D1 Limits Polymorphonuclear Leukocyte Recruitment to Inflammatory Loci : Receptor-Dependent Actions. Lucy V. Norling et al, Arteriosclerosis, Thrombosis, and Vascular Biology. 2012; 32: 1970-1978

    Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Carmela De Santo et al, Nature Immunology VOLUME 11 NUMBER 11 NOVEMBER 2010

    If you need any further assistance in the future, please do not hesitate to contact me.

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    Question

    Inquiry: Hello, I was wondering if you could tell me what it means when it says the antibody is pre absorbed? Also can you tell me if this is compatible as a secondary for the the antibody ab26316.

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    Answer

    Thank you for contacting us.

    Antibodies are sometimes pre-adsorbed. This means they have been adsorbed with other proteins, or serum from various species, etc, to eliminate any antibody that may cross-react. The resulting purified antibody should be very pure and specific and any cross reactivity should be significantly reduced.

    As shown on the datasheet, the primary antibody ab26316 is a Mouse IgG1. Therefore I confirm that ab99605 is compatible with ab26316.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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