Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
![Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)](https://www.abcam.com/ps/products/26/ab26316/Images/ab26316-37208-anti-fprl1-antibody-gm1d6-flow-cytometry.jpg "Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)")
Key features and details
- Mouse monoclonal [GM1D6] to FPRL1/RFP
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FPRL1/RFP antibody [GM1D6]
See all FPRL1/RFP primary antibodies -
Description
Mouse monoclonal [GM1D6] to FPRL1/RFP -
Host species
Mouse -
Specificity
Ab26316 recognises FPRL1/RFP transiently expressed on the cell surface of transfected BOSC cells.
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Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: BOSC23 cells (Human) ICC: CHO cells transfected with an expession vector encoding FPRL1/RFP
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
GM1D6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use 1µg for 106 cells.
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| Flow Cyt |
Use 1.2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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ICC/IF
Use 1µg for 106 cells. |
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Flow Cyt
Use 1.2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). -
Tissue specificity
Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2358 Human
- Omim: 136538 Human
- SwissProt: P25090 Human
- Unigene: 99855 Human
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Alternative names
- ALXR antibody
- FMLP-R-I antibody
- FMLP-R-II antibody
see all
Images
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Flow Cytometry - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)
FACS analysis of BOSC23 cells using ab26316. BOSC23 cells were transiently transfected with with an expression vector containing either FPRL1/RFP (red curve) or a control transfectant (black curve). Binding of ab26316 was detected using a PE-conjugated secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)](https://www.abcam.com/ps/products/26/ab26316/Images/ab26316-37209-anti-fprl1-antibody-gm1d6-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FPRL1/RFP antibody [GM1D6] (ab26316)ab26316 staining of FPRL1/RFP in CHO cells using 1µg/106 cells. CHO cells were transiently transfected with an expession vector encoding FPRL1/RFP and binding of ab26316 was visualized using a FITC-conjugated secondary antibody (green). Actin filaments are labelled with Alexa Fluor-55 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab26316 has been referenced in 8 publications.
- Yu Y et al. The G-protein-coupled chemoattractant receptor Fpr2 exacerbates neuroglial dysfunction and angiogenesis in diabetic retinopathy. FASEB Bioadv 2:613-623 (2020). PubMed: 33089077
- Dakin SG et al. 15-Epi-LXA4 and MaR1 counter inflammation in stromal cells from patients with Achilles tendinopathy and rupture. FASEB J N/A:fj201900196R (2019). PubMed: 30916999
- Dakin SG et al. Proresolving Mediators LXB4 and RvE1 Regulate Inflammation in Stromal Cells from Patients with Shoulder Tendon Tears. Am J Pathol 189:2258-2268 (2019). PubMed: 31437425
- Herteman N et al. Characterization of Circulating Low-Density Neutrophils Intrinsic Properties in Healthy and Asthmatic Horses. Sci Rep 7:7743 (2017). IF ; Horse . PubMed: 28798364
- Hsiao HM et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). PubMed: 25320283
- Dakin SG et al. Macrophage sub-populations and the lipoxin A4 receptor implicate active inflammation during equine tendon repair. PLoS One 7:e32333 (2012). IHC-Fr ; Horse . PubMed: 22384219
- Dakin SG et al. Inflamm-aging and arachadonic acid metabolite differences with stage of tendon disease. PLoS One 7:e48978 (2012). IHC-Fr ; Horse . PubMed: 23155437
- Björkman L et al. Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. J Leukoc Biol 83:245-53 (2008). PubMed: 17984291
