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    fra1-antibody-ep4711-ab124722.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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RecombinantRabMAb

Recombinant Anti-FRA1 antibody [EP4711] (ab124722)

  • Datasheet
  • SDS
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Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
  • Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)
  • Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
  • OI-RD Scanning - Anti-FRA1 antibody [EP4711] (ab124722)
  • Anti-FRA1 antibody [EP4711] (ab124722)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4711] to FRA1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant Human FRA1 protein (ab82119)

View more associated products

Overview

  • Product name

    Anti-FRA1 antibody [EP4711]
    See all FRA1 primary antibodies
  • Description

    Rabbit monoclonal [EP4711] to FRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human FRA1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HL60, HeLa and T47D cell lysates; A431 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.22 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP4711
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-FRA1 antibody [EP4711] (ab225129)
    • Alexa Fluor® 488 Anti-FRA1 antibody [EP4711] (ab225130)
    • Anti-FRA1 antibody [EP4711] - BSA and Azide free (ab226086)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • T47D nuclear extract lysate (ab14896)
  • Recombinant Protein

    • Recombinant Human FRA1 protein (ab82119)

Applications

Our Abpromise guarantee covers the use of ab124722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Sequence similarities

      Belongs to the bZIP family. Fos subfamily.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P15407 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8061 Human
      • Omim: 136515 Human
      • SwissProt: P15407 Human
      • Unigene: 283565 Human
      • Alternative names

        • FOS L1 antibody
        • FOS like antigen 1 antibody
        • Fos related antigen 1 antibody
        • Fos-related antigen 1 antibody
        • FOSL 1 antibody
        • FOSL1 antibody
        • FOSL1 protein antibody
        • FOSL1_HUMAN antibody
        • FRA 1 antibody
        • FRA-1 antibody
        see all

      Images

      • Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution

        Lane 1 : HL60 cell lysate
        Lane 2 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)
        Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)

        ab124722, at 1/250 dilution, staining FRA1 in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.

      • Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution

        Lane 1 : T47D cell lysate
        Lane 2 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa

      • OI-RD Scanning - Anti-FRA1 antibody [EP4711] (ab124722)
        OI-RD Scanning - Anti-FRA1 antibody [EP4711] (ab124722)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-FRA1 antibody [EP4711] (ab124722)
        Anti-FRA1 antibody [EP4711] (ab124722)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (5)

      Publishing research using ab124722? Please let us know so that we can cite the reference in this datasheet.

      ab124722 has been referenced in 5 publications.

      • Lin C  et al. Importance of PNO1 for growth and survival of urinary bladder carcinoma: Role in core-regulatory circuitry. J Cell Mol Med 24:1504-1515 (2020). PubMed: 31800162
      • Yang Y  et al. The effect of Helicobacter pylori on the expression of FRA-1 in gastric epithelial cells and its mechanism. Microb Pathog 129:257-265 (2019). PubMed: 30807813
      • Guo J  et al. Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis. World J Gastroenterol 25:6386-6403 (2019). PubMed: 31798276
      • Zadora PK  et al. Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells. Cell Rep 26:1286-1302.e8 (2019). PubMed: 30699355
      • Qiu L  et al. Oncogenic Activation of Nrf2, Though as a Master Antioxidant Transcription Factor, Liberated by Specific Knockout of the Full-Length Nrf1a that Acts as a Dominant Tumor Repressor. Cancers (Basel) 10:N/A (2018). PubMed: 30562963

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab124722.
      Please use the links above to contact us or submit feedback about this product.

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