Recombinant
RabMAb

Recombinant Anti-FRA1 antibody [EP4711] (ab124722)

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Overview

  • Product name

    Anti-FRA1 antibody [EP4711]
    See all FRA1 primary antibodies

  • Description

    Rabbit monoclonal [EP4711] to FRA1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FRA1 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HL60, HeLa and T47D cell lysates; A431 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Sequence similarities

      Belongs to the bZIP family. Fos subfamily.
      Contains 1 bZIP domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • FOS L1 antibody
        • FOS like antigen 1 antibody
        • Fos related antigen 1 antibody
        • Fos-related antigen 1 antibody
        • FOSL 1 antibody
        • FOSL1 antibody
        • FOSL1 protein antibody
        • FOSL1_HUMAN antibody
        • FRA 1 antibody
        • FRA-1 antibody
        see all

      Images

      • Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution

        Lane 1 : HL60 cell lysate
        Lane 2 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)
        Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)

        ab124722, at 1/250 dilution, staining FRA1 in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.

      • Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        Western blot - Anti-FRA1 antibody [EP4711] (ab124722)
        All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution

        Lane 1 : T47D cell lysate
        Lane 2 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa

      • Other - Anti-FRA1 antibody [EP4711] (ab124722)
        Other - Anti-FRA1 antibody [EP4711] (ab124722)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Yang Y  et al. The effect of Helicobacter pylori on the expression of FRA-1 in gastric epithelial cells and its mechanism. Microb Pathog 129:257-265 (2019). Read more (PubMed: 30807813) »
      • Qiu L  et al. Oncogenic Activation of Nrf2, Though as a Master Antioxidant Transcription Factor, Liberated by Specific Knockout of the Full-Length Nrf1a that Acts as a Dominant Tumor Repressor. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30562963) »
      See all 2 Publications for this product

      Publishing research using ab124722? Please let us know so that we can cite the reference in this datasheet.

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