Recombinant Anti-FRA1 antibody [EP4711] (ab124722)
![Western blot - Anti-FRA1 antibody [EP4711] (ab124722)](https://www.abcam.com/ps/products/124/ab124722/Images/ab124722-138540-anti-fra1-antibody-ep4711-western-blot.jpg "Western blot - Anti-FRA1 antibody [EP4711] (ab124722)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4711] to FRA1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-FRA1 antibody [EP4711]
See all FRA1 primary antibodies -
Description
Rabbit monoclonal [EP4711] to FRA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FRA1 aa 150-250. The exact sequence is proprietary.
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Positive control
- HL60, HeLa and T47D cell lysates; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.22 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP4711 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 29 kDa). | |
| ICC/IF | 1/250 - 1/500. |
Target
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Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8061 Human
- Omim: 136515 Human
- SwissProt: P15407 Human
- Unigene: 283565 Human
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Alternative names
- FOS L1 antibody
- FOS like antigen 1 antibody
- Fos related antigen 1 antibody
see all
Images
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Western blot - Anti-FRA1 antibody [EP4711] (ab124722)All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)](https://www.abcam.com/ps/products/124/ab124722/Images/ab124722-138539-anti-fra1-antibody-ep4711-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FRA1 antibody [EP4711] (ab124722)ab124722, at 1/250 dilution, staining FRA1 in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.
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![Western blot - Anti-FRA1 antibody [EP4711] (ab124722)](https://www.abcam.com/ps/products/124/ab124722/Images/ab124722-138538-anti-fra1-antibody-ep4711-western-blot.jpg)
Western blot - Anti-FRA1 antibody [EP4711] (ab124722)All lanes : Anti-FRA1 antibody [EP4711] (ab124722) at 1/1000 dilution
Lane 1 : T47D cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
![OI-RD Scanning - Anti-FRA1 antibody [EP4711] (ab124722)](https://www.abcam.com/ps/products/124/ab124722/Images/ab124722-138537-anti-fra1-antibody-ep4711.jpg)
OI-RD Scanning - Anti-FRA1 antibody [EP4711] (ab124722) -
![Anti-FRA1 antibody [EP4711] (ab124722)](https://www.abcam.com/ps/products/124/ab124722/Images/ab124722-5-benefits-of-recombinant-antibodies.png)
Anti-FRA1 antibody [EP4711] (ab124722)
Protocols
References (5)
ab124722 has been referenced in 5 publications.
- Lin C et al. Importance of PNO1 for growth and survival of urinary bladder carcinoma: Role in core-regulatory circuitry. J Cell Mol Med 24:1504-1515 (2020). PubMed: 31800162
- Yang Y et al. The effect of Helicobacter pylori on the expression of FRA-1 in gastric epithelial cells and its mechanism. Microb Pathog 129:257-265 (2019). PubMed: 30807813
- Guo J et al. Ubiquitin-conjugating enzyme E2T knockdown suppresses hepatocellular tumorigenesis via inducing cell cycle arrest and apoptosis. World J Gastroenterol 25:6386-6403 (2019). PubMed: 31798276
- Zadora PK et al. Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells. Cell Rep 26:1286-1302.e8 (2019). PubMed: 30699355
- Qiu L et al. Oncogenic Activation of Nrf2, Though as a Master Antioxidant Transcription Factor, Liberated by Specific Knockout of the Full-Length Nrf1a that Acts as a Dominant Tumor Repressor. Cancers (Basel) 10:N/A (2018). PubMed: 30562963
