Recombinant Anti-FRA2 antibody [EPR4713(2)] (ab124830)
![Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)](https://www.abcam.com/ps/products/124/ab124830/Images/ab124830-138778-anti-fra2-antibody-epr47132-western-blot.jpg "Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4713(2)] to FRA2
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FRA2 antibody [EPR4713(2)]
See all FRA2 primary antibodies -
Description
Rabbit monoclonal [EPR4713(2)] to FRA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FRA2 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T cells; MCF7, F9, PC12, NCCIT, and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4713(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).
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| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa). |
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Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Application notes
Is unsuitable for ICC or IHC-P.
Target
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Function
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts. -
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2355 Human
- Entrez Gene: 14284 Mouse
- Entrez Gene: 25446 Rat
- Omim: 601575 Human
- SwissProt: P15408 Human
- SwissProt: P47930 Mouse
- SwissProt: P51145 Rat
- Unigene: 220971 Human
see all -
Alternative names
- FLJ23306 antibody
- Fos L2 antibody
- FOS like antigen 2 antibody
see all
Images
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Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)All lanes : Anti-FRA2 antibody [EPR4713(2)] (ab124830) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : F9 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NCCIT cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)](https://www.abcam.com/ps/products/124/ab124830/Images/ab124830-138777-anti-fra2-antibody-epr47132-flow-cytometry.jpg)
Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)ab124830 at 1/100 dilution staining FRA2 in permeabilized 293T cells (red). A rabbit IgG was used as negative control (green). -
![Anti-FRA2 antibody [EPR4713(2)] (ab124830)](https://www.abcam.com/ps/products/124/ab124830/Images/ab124830-3-benefits-of-recombinant-antibodies.png)
Anti-FRA2 antibody [EPR4713(2)] (ab124830)
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab124830 has been referenced in 5 publications.
- Song LN et al. Hsa_circ_0003998 promotes epithelial to mesenchymal transition of hepatocellular carcinoma by sponging miR-143-3p and PCBP1. J Exp Clin Cancer Res 39:114 (2020). PubMed: 32552766
- Ren W et al. Calcitonin gene-related peptide regulates FOSL2 expression and cell proliferation of BMSCs via mmu_circRNA_003795. Mol Med Rep 19:3732-3742 (2019). PubMed: 30896827
- Li Z et al. lncRNA UCA1 Mediates Resistance to Cisplatin by Regulating the miR-143/FOSL2-Signaling Pathway in Ovarian Cancer. Mol Ther Nucleic Acids 17:92-101 (2019). PubMed: 31234009
- He J et al. miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncol Rep 37:2672-2678 (2017). WB ; Human . PubMed: 28393251
- Renaud SJ et al. The FOS transcription factor family differentially controls trophoblast migration and invasion. J Biol Chem 289:5025-39 (2014). WB ; Human . PubMed: 24379408