Anti-FRA2 antibody [EPR4713(2)] (ab124830)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-FRA2 antibody [EPR4713(2)]
See all FRA2 primary antibodies -
DescriptionRabbit monoclonal [EPR4713(2)] to FRA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human FRA2 aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T cells; MCF7, F9, PC12, NCCIT, and 293T cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4713(2)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionControls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts.
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Sequence similaritiesBelongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2355 Human
- Entrez Gene: 14284 Mouse
- Entrez Gene: 25446 Rat
- Omim: 601575 Human
- SwissProt: P15408 Human
- SwissProt: P47930 Mouse
- SwissProt: P51145 Rat
- Unigene: 220971 Human
see all -
Alternative names
- FLJ23306 antibody
- Fos L2 antibody
- FOS like antigen 2 antibody
see all
Images
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![Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)](https://www.abcam.com/ps/datasheet/images/124/ab124830/FRA2-Primary-antibodies-ab124830-1.jpg)
Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)All lanes : Anti-FRA2 antibody [EPR4713(2)] (ab124830) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : F9 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NCCIT cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)](https://www.abcam.com/ps/datasheet/images/124/ab124830/FRA2-Primary-antibodies-ab124830-3.jpg)
Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)ab124830 at 1/100 dilution staining FRA2 in permeabilized 293T cells (red). A rabbit IgG was used as negative control (green).
References
This product has been referenced in:
- He J et al. miR-597 inhibits breast cancer cell proliferation, migration and invasion through FOSL2. Oncol Rep 37:2672-2678 (2017). WB ; Human . Read more (PubMed: 28393251) »
- Renaud SJ et al. The FOS transcription factor family differentially controls trophoblast migration and invasion. J Biol Chem 289:5025-39 (2014). WB ; Human . Read more (PubMed: 24379408) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"