Recombinant
RabMAb

Recombinant Anti-FRA2 antibody [EPR4713(2)] (ab124830)

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Overview

  • Product name

    Anti-FRA2 antibody [EPR4713(2)]
    See all FRA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4713(2)] to FRA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human FRA2 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T cells; MCF7, F9, PC12, NCCIT, and 293T cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab124830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    Images

    • Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)
      Western blot - Anti-FRA2 antibody [EPR4713(2)] (ab124830)
      All lanes : Anti-FRA2 antibody [EPR4713(2)] (ab124830) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : F9 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NCCIT cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)
      Flow Cytometry - Anti-FRA2 antibody [EPR4713(2)] (ab124830)
      ab124830 at 1/100 dilution staining FRA2 in permeabilized 293T cells (red). A rabbit IgG was used as negative control (green).

    References

    This product has been referenced in:

    • Ren W  et al. Calcitonin gene-related peptide regulates FOSL2 expression and cell proliferation of BMSCs via mmu_circRNA_003795. Mol Med Rep 19:3732-3742 (2019). Read more (PubMed: 30896827) »
    • Li Z  et al. lncRNA UCA1 Mediates Resistance to Cisplatin by Regulating the miR-143/FOSL2-Signaling Pathway in Ovarian Cancer. Mol Ther Nucleic Acids 17:92-101 (2019). Read more (PubMed: 31234009) »
    See all 4 Publications for this product

    Publishing research using ab124830? Please let us know so that we can cite the reference in this datasheet.

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